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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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AMY2B Antibody (aa74‑100) LS‑C156320 Best Seller
AMY2B antibody LS-C156320 is an unconjugated rabbit polyclonal antibody to human AMY2B. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C156320-400
400 µl
Unavailable

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Product Description

AMY2B antibody LS-C156320 is an unconjugated rabbit polyclonal antibody to human AMY2B. Validated for Flow, IF, IHC and WB.
About AMY2B
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. P19961 NM_020978 NP_066188.1

AMY2B Antibody, Alpha-amylase 2B Antibody, Amylase, alpha 2B (pancreatic) Antibody, Amylase, alpha 2B pancreatic Antibody, Carcinoid alpha-amylase Antibody, Glycogenase Antibody, Alpha-amylase carcinoid Antibody, AMY2 Antibody


Specifications

Target
Human AMY2B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E415 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156320. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa74-100
Specificity
This AMY2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the N-terminal region of human AMY2B.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

AMY2B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
AMY2B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of AMY2B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of AMY2B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

AMY2B Antibody western blot of HL-60,CEM and K562 cell line lysates (35 ug/lane). The AMY2B antibody detected the AMY2B protein (arrow).
AMY2B Antibody western blot of HL-60,CEM and K562 cell line lysates (35 ug/lane). The AMY2B antibody detected the AMY2B protein (arrow).

Flow Cytometry

AMY2B Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AMY2B Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

AMY2B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
AMY2B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of AMY2B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of AMY2B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

AMY2B Antibody western blot of HL-60,CEM and K562 cell line lysates (35 ug/lane). The AMY2B antibody detected the AMY2B protein (arrow).
AMY2B Antibody western blot of HL-60,CEM and K562 cell line lysates (35 ug/lane). The AMY2B antibody detected the AMY2B protein (arrow).

Flow Cytometry

AMY2B Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AMY2B Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

AMY2B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
AMY2B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of AMY2B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of AMY2B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

AMY2B Antibody western blot of HL-60,CEM and K562 cell line lysates (35 ug/lane). The AMY2B antibody detected the AMY2B protein (arrow).
AMY2B Antibody western blot of HL-60,CEM and K562 cell line lysates (35 ug/lane). The AMY2B antibody detected the AMY2B protein (arrow).

Flow Cytometry

AMY2B Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AMY2B Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

AMY2B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
AMY2B antibody immunohistochemistry of formalin-fixed and paraffin-embedded human brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Confocal immunofluorescence of AMY2B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of AMY2B Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

AMY2B Antibody western blot of HL-60,CEM and K562 cell line lysates (35 ug/lane). The AMY2B antibody detected the AMY2B protein (arrow).
AMY2B Antibody western blot of HL-60,CEM and K562 cell line lysates (35 ug/lane). The AMY2B antibody detected the AMY2B protein (arrow).

Flow Cytometry

AMY2B Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AMY2B Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/22/2018

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