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AMY2A / Pancreatic Amylase Antibody (aa156‑430) LS‑C186280
Pancreatic Amylase antibody LS-C186280 is an unconjugated rabbit polyclonal antibody to human Pancreatic Amylase (AMY2A). Validated for IHC and WB.
Catalog
Size
Price
LS-C186280-100
100 µl (1 mg/ml)
Unavailable

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Product Description

Pancreatic Amylase antibody LS-C186280 is an unconjugated rabbit polyclonal antibody to human Pancreatic Amylase (AMY2A). Validated for IHC and WB.
About AMY2A / Pancreatic Amylase
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. P04746 NM_000699 NP_000690.1

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Specifications

Target
Human AMY2A / Pancreatic Amylase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant fragment corresponding to a region within amino acids 156 and 430 of alpha amylase 2A (pancreatic) (SwissProt P04746). Percent identity by BLAST analysis: Human (100%); Bovine (89%).
Epitope
aa156-430
Specificity
Human AMY2A / Pancreatic Amylase
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded OVCA using alpha amylase 2A (pancreatic) antibody at 1:500 dilution.
IHC of paraffin-embedded OVCA using alpha amylase 2A (pancreatic) antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Molt-4 , B: Raji. 7.5% SDS PAGE. AMY2A / Pancreatic Amylase antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Molt-4 , B: Raji. 7.5% SDS PAGE. AMY2A / Pancreatic Amylase antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded OVCA using alpha amylase 2A (pancreatic) antibody at 1:500 dilution.
IHC of paraffin-embedded OVCA using alpha amylase 2A (pancreatic) antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Molt-4 , B: Raji. 7.5% SDS PAGE. AMY2A / Pancreatic Amylase antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Molt-4 , B: Raji. 7.5% SDS PAGE. AMY2A / Pancreatic Amylase antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded OVCA using alpha amylase 2A (pancreatic) antibody at 1:500 dilution.
IHC of paraffin-embedded OVCA using alpha amylase 2A (pancreatic) antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Molt-4 , B: Raji. 7.5% SDS PAGE. AMY2A / Pancreatic Amylase antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Molt-4 , B: Raji. 7.5% SDS PAGE. AMY2A / Pancreatic Amylase antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded OVCA using alpha amylase 2A (pancreatic) antibody at 1:500 dilution.
IHC of paraffin-embedded OVCA using alpha amylase 2A (pancreatic) antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A: Molt-4 , B: Raji. 7.5% SDS PAGE. AMY2A / Pancreatic Amylase antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Molt-4 , B: Raji. 7.5% SDS PAGE. AMY2A / Pancreatic Amylase antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/22/2018

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