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Anti-AMPH / Amphiphysin Antibody (clone 3) IHC-plus™ LS-B3757

Note: This antibody replaces LS-C63306

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml Unavailable

Most Popular AMPH / Amphiphysin Antibodies

Anti-AMPH / Amphiphysin Antibody (aa159-170) IHC-plus™ LS-B2760
Goat Polyclonal to Human AMPH / Amphiphysin
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-AMPH / Amphiphysin Antibody (clone 3) IHC-plus™ LS-B3757
Mouse Monoclonal [clone 3] (IgG) to Human AMPH / Amphiphysin
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-AMPH / Amphiphysin Antibody (aa1-347) IHC-plus™ LS-B7822
Mouse Monoclonal (IgG1,k) to Human AMPH / Amphiphysin
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 3] (IgG) to Human AMPH / Amphiphysin
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human AMPH / Amphiphysin
Mouse
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit (tested or 100% immunogen sequence identity)
IgG Monoclonal [3]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.5 µg/ml)
  • Immunoprecipitation

Specificity and Use

AMPH / Amphiphysin antibody was raised against recombinant human Amphiphysin.
Detects a ~128 kD protein, corresponding to the apparent molecular mass of Amphiphysin I on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, canine, hamster, monkey and rabbit origins. A 108 kD isoform may be observed in some tissues or cell lysates. The epitope is located within the carboxyl terminal region of Amphiphysin I (residues 545-695). Rat brain immunostaining using this antibody in immunofluorescence shows synaptic distribution.
Immunohistochemistry: LS-B3757 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3757 was determined to be 10 ug/ml.

Packaging

PBS, 0.1 mM PMSF, 50% glycerol. Sourced in Ascites.
Store at -20°C.
For research use only.

About AMPH / Amphiphysin

P49418 NM_001635 NP_001626.1

AMPH Antibody, AMPH1 Antibody, Amphiphysin Antibody, Amphiphysin I Antibody

AMPH / Amphiphysin is a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.

Immunohistochemistry

Immunohistochemistry
Anti-AMPH / Amphiphysin antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3757 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3757 concentration 10 ug/ml.

Requested From: 
Date Requested: 3/28/2017

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