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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AMIGO2 Antibody (aa290‑339) LS‑C135358
AMIGO2 antibody LS-C135358 is an unconjugated rabbit polyclonal antibody to AMIGO2 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C135358-50
50 µg
Unavailable

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Immunohistochemistry Dilution at 1:800 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
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Product Description

AMIGO2 antibody LS-C135358 is an unconjugated rabbit polyclonal antibody to AMIGO2 from human, mouse, rat and other species. Validated for WB.
About AMIGO2
AMIGO2/ALIVIN-1 is a member of a small family of type I membrane proteins containing six leucine rich repeats (LRR), one immunoglobulin domain and cysteine rich LLR -NH2 and -COOH domains. In assays a substrate bound form of AMIGO2 promoted prominent neurite extension and inhibited fasciculation of neurites. A homophilic and heterophilic binding mechanism exists between members of the AMIGO family. Q86SJ2 NM_181847 NP_862830.1

AMIGO2 Antibody, ALI1 Antibody, Alivin 1 Antibody, Alivin-1 Antibody, Alvin 1 Antibody, AMIGO-2 Antibody, Amphoterin induced gene 2 Antibody, Amphoterin-induced protein 2 Antibody, Transmembrane protein AMIGO2 Antibody, DEGA Antibody


Specifications

Target
Rat AMIGO2
Host
Rabbit
Reactivity
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Hamster, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Guinea pig, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
AMIGO2 antibody was raised against synthetic peptide located between aa290-339 of rat Amigo2 (Q7TNJ4, NP_877968). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Pig (100%); Horse, Guinea pig (92%); Rabbit, Lizard (84%).
Blocking Peptide
LS-E20194 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135358. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa290-339
Specificity
Rat AMIGO2
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

AMIGO2 antibody LS-C135358 Western blot of Rat Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AMIGO2 antibody LS-C135358 Western blot of Rat Heart lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

AMIGO2 antibody LS-C135358 Western blot of Rat Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AMIGO2 antibody LS-C135358 Western blot of Rat Heart lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

AMIGO2 antibody LS-C135358 Western blot of Rat Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AMIGO2 antibody LS-C135358 Western blot of Rat Heart lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

AMIGO2 antibody LS-C135358 Western blot of Rat Heart lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AMIGO2 antibody LS-C135358 Western blot of Rat Heart lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/19/2018

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