LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-ALPL / Alkaline Phosphatase Antibody IHC-plus™ LS-B3666

Note: This antibody replaces LS-C17269

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ALPL / Alkaline Phosphatase Antibodies

Anti-ALPL / Alkaline Phosphatase Antibody (clone TRA-2-49/6E) LS-C38190
Mouse Monoclonal [clone TRA-2-49/6E] (IgG1) to Human ALPL / Alkaline Phosphatase
Human, Monkey, Cat, Pig, Rabbit
Immunofluorescence, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-ALPL / Alkaline Phosphatase Antibody IHC-plus™ LS-B3666
Mouse Monoclonal (IgG1) to Human ALPL / Alkaline Phosphatase
Human, Bovine
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human ALPL / Alkaline Phosphatase
Human, Bovine
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ALPL / Alkaline Phosphatase
Mouse
Human, Bovine (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

ALPL / Alkaline Phosphatase antibody was raised against purified alkaline phosphatase (native) from human bone.
Recognizes Alkaline Phosphatase from human bone. Species cross-reactivity: Alkaline phosphatase from human placenta and calf intestine.
Immunohistochemistry: LS-B3666 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3666 was determined to be 10 ug/ml. Suitable for use in ELISA and Western Blot. Western Blot: Stains a band of bone AP at ~90kD.

Packaging

PBS, pH 7.2, 0.09% sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ALPL / Alkaline Phosphatase

P05186 NM_000478 NP_000469.3

ALPL Antibody, Alkaline phosphomonoesterase Antibody, AP-TNAP Antibody, Alkaline phosphatase liver Antibody, APTNAP Antibody, Glycerophosphatase Antibody, Tissue-nonspecific ALP Antibody, TNAP Antibody, HOPS Antibody, Liver alkaline phosphatase Antibody, TNSALP Antibody

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme.

Immunohistochemistry

Immunohistochemistry
Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.

Requested From: United States
Date Requested: 12/7/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number