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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ALPL / Alkaline Phosphatase Antibody IHC‑plus™ LS‑B3666
Note: This antibody replaces LS-C17269
Alkaline Phosphatase antibody LS-B3666 is an unconjugated mouse monoclonal antibody to Alkaline Phosphatase (ALPL) from human and bovine. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B3666-50
50 µg (1 mg/ml)
Unavailable

Popular ALPL / Alkaline Phosphatase Products

Antibody
Species: Human, Monkey, Cat, Pig, Rabbit
Applications: Immunofluorescence, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
Antibody
Species: Human, Mouse
Applications: Western blot, ELISA

Product Description

Alkaline Phosphatase antibody LS-B3666 is an unconjugated mouse monoclonal antibody to Alkaline Phosphatase (ALPL) from human and bovine. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About ALPL / Alkaline Phosphatase
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. P05186 NM_000478 NP_000469.3

ALPL Antibody, Alkaline phosphomonoesterase Antibody, AP-TNAP Antibody, Alkaline phosphatase liver Antibody, APTNAP Antibody, Glycerophosphatase Antibody, Tissue-nonspecific ALP Antibody, TNAP Antibody, HOPS Antibody, Liver alkaline phosphatase Antibody, TNSALP Antibody


Specifications

Target
Human ALPL / Alkaline Phosphatase
Host
Mouse
Reactivity
Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified alkaline phosphatase (native) from human bone.
Specificity
Recognizes Alkaline Phosphatase from human bone. Species cross-reactivity: Alkaline phosphatase from human placenta and calf intestine.
Usage
Immunohistochemistry: LS-B3666 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3666 was determined to be 10 ug/ml. Suitable for use in ELISA and Western Blot. Western Blot: Stains a band of bone AP at ~90kD.
Presentation
PBS, pH 7.2, 0.09% sodium azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.

Immunohistochemistry

Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.

Immunohistochemistry

Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.

Immunohistochemistry

Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.

Immunohistochemistry

Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.

Immunohistochemistry

Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.

Immunohistochemistry

Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.

Immunohistochemistry

Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.
Anti-Bone Alkaline Phosphatase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3666 concentration 10 ug/ml.


Requested From: 
Date Requested: 6/24/2018

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