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| Catalog Number | Size | Price |
|---|---|---|
| LS-C59247-2 | 2 ml (85 mg/ml) | $1,225 |
![ALPI / Alkaline Phosphatase Antibody - Anti-Alkaline Phosphatase Antibody - Western Blot. Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (LS-C60884) diluted 1:4000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/alpi-alkaline-phosphatase-antibody-ls-c59247/62504_59751.jpg)
Polyclonal Rabbit anti‑Human ALPI / Alkaline Phosphatase Antibody (WB) LS‑C59247
Polyclonal Rabbit anti‑Human ALPI / Alkaline Phosphatase Antibody (WB) LS‑C59247
Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Alkaline Phosphatase antibody LS-C59247 is an unconjugated rabbit polyclonal antibody to human Alkaline Phosphatase (ALPI). Validated for ELISA and WB.
Target
Human ALPI / Alkaline Phosphatase
Synonyms
ALPI | Alkaline phosphomonoesterase | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
Alkaline Phosphatase [human intestine].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase [Human Intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:5000 - 1:20000)
Usage
Suitable for immunoblotting (western or dot blot), ELISA and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of Alkaline Phosphatase [Human Intestine] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884 and ABTS as a substrate for 30 minutes at room temperature.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ALPI / Alkaline Phosphatase
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Species:
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Species:
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Applications:
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Applications:
Western blot, ELISA
Species:
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Applications:
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
