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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ALPI / Alkaline Phosphatase Antibody LS‑C38202
Alkaline Phosphatase antibody LS-C38202 is an unconjugated sheep polyclonal antibody to bovine Alkaline Phosphatase (ALPI). Validated for ELISA, Flow, IP and WB.
Antibody
Catalog
Size
Price
LS-C38202-100
100 µg
Unavailable

Popular ALPI / Alkaline Phosphatase Products

Anti-ALPI / Alkaline Phosphatase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B326 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemical of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Western blot using ALPI monoclonal antibody against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.
Species: Human
Applications: Flow Cytometry, ELISA
Antibody
Species: Bovine
Applications: IHC, Western blot, ELISA
Western blot of Intestinal-type alkaline phosphatase antibody at 2ug/ml + 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 58 kDa. Observed band size: 58 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

Alkaline Phosphatase antibody LS-C38202 is an unconjugated sheep polyclonal antibody to bovine Alkaline Phosphatase (ALPI). Validated for ELISA, Flow, IP and WB.
About ALPI / Alkaline Phosphatase
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. P09923 NM_001631 NP_001622.2

ALPI Antibody, Alkaline phosphomonoesterase Antibody, Kasahara isozyme Antibody, Glycerophosphatase Antibody, Alkaline Phosphatase Antibody, IAP Antibody


Specifications

Target
Bovine ALPI / Alkaline Phosphatase
Host
Sheep
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA (1:1000)
Immunogen
Alkaline Phosphatase from calf intestine.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified alkaline phosphatase [calf intestine] and anti-sheep serum. Cross-reactivity against alkaline phosphatase from other tissues and species may occur but have not been specifically determined.
Usage
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation. ELISA: 1:1000 to 1:5000.
Presentation
PBS, pH 7.2. No preservative added.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 7/19/2018

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