LSBio
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Immunohistochemistry Reagents
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Research Areas
  • Cancer Research Resources and Products:
  • Breast Cancer
  • Colorectal Cancer
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Reviews
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-ALPI / Alkaline Phosphatase Antibody (clone 3A8) LS-C196649

Catalog Size Price
LS-C196649-100 100 µg Unavailable

Most Popular ALPI / Alkaline Phosphatase Antibodies

Anti-ALPI / Alkaline Phosphatase Antibody IHC-plus™ LS-B326
Rabbit Polyclonal to Human ALPI / Alkaline Phosphatase
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-ALPI / Alkaline Phosphatase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B326 concentration 5 ug/ml.
Anti-ALPI / Alkaline Phosphatase Antibody (aa274-304) LS-C98228
Rabbit Polyclonal to Human ALPI / Alkaline Phosphatase
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Anti-ALPI / Alkaline Phosphatase Antibody (aa397-458, clone 3B10E7) LS-C191827
Mouse Monoclonal [clone 3B10E7] (IgG1) to Human ALPI / Alkaline Phosphatase
Human
Flow Cytometry, ELISA
Unconjugated
Western blot using ALPI monoclonal antibody against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.
Anti-ALPI / Alkaline Phosphatase Antibody (HRP) LS-C209238
Rabbit Polyclonal to Bovine ALPI / Alkaline Phosphatase
Bovine
IHC, Western blot, ELISA
HRP Conjugated
Anti-ALPI / Alkaline Phosphatase Antibody LS-C211435
Rabbit Polyclonal (IgG) to Human ALPI / Alkaline Phosphatase
Human
IHC, Western blot, ELISA
Unconjugated
Western blot of Intestinal-type alkaline phosphatase antibody at 2ug/ml + 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 58 kDa. Observed band size: 58 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed
Mouse Monoclonal [clone 3A8] (IgG2a,k) to Human ALPI / Alkaline Phosphatase
Human
Western blot, ELISA
Unconjugated

Details

Human ALPI / Alkaline Phosphatase
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [3A8]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Western blot
  • ELISA

Specificity and Use

ALPI (NP_001622, aa74-162 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human ALPI / Alkaline Phosphatase
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
For research use only.

About ALPI / Alkaline Phosphatase

P09923 NM_001631 NP_001622.2

ALPI Antibody, Alkaline phosphomonoesterase Antibody, Kasahara isozyme Antibody, Glycerophosphatase Antibody, Alkaline Phosphatase Antibody, IAP Antibody

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.

Western blot

ALPI monoclonal antibody (M03), clone 3A8 Western Blot analysis of ALPI expression in HeLa.
ALPI monoclonal antibody (M03), clone 3A8 Western Blot analysis of ALPI expression in HeLa.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ALPI is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ALPI is 0.3 ng/ml as a capture antibody.

Requested From: 
Date Requested: 5/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number