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Anti-ALPI / Alkaline Phosphatase Antibody (aa274-304) LS-C98228

Catalog Size Price
LS-C98228-400 400 µl Unavailable

Most Popular ALPI / Alkaline Phosphatase Antibodies

Anti-ALPI / Alkaline Phosphatase Antibody IHC-plus™ LS-B326
Rabbit Polyclonal to Human ALPI / Alkaline Phosphatase
Human
IHC, IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-ALPI / Alkaline Phosphatase antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B326 concentration 5 ug/ml.
Anti-ALPI / Alkaline Phosphatase Antibody (aa274-304) LS-C98228
Rabbit Polyclonal to Human ALPI / Alkaline Phosphatase
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Anti-ALPI / Alkaline Phosphatase Antibody (aa397-458, clone 3B10E7) LS-C191827
Mouse Monoclonal [clone 3B10E7] (IgG1) to Human ALPI / Alkaline Phosphatase
Human
Flow Cytometry, ELISA
Unconjugated
Western blot using ALPI monoclonal antibody against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.
Anti-ALPI / Alkaline Phosphatase Antibody (HRP) LS-C209238
Rabbit Polyclonal to Bovine ALPI / Alkaline Phosphatase
Bovine
IHC, Western blot, ELISA
HRP Conjugated
Anti-ALPI / Alkaline Phosphatase Antibody LS-C211435
Rabbit Polyclonal (IgG) to Human ALPI / Alkaline Phosphatase
Human
IHC, Western blot, ELISA
Unconjugated
Western blot of Intestinal-type alkaline phosphatase antibody at 2ug/ml + 293T whole cell lysate. Secondary: Goat polyclonal to Rabbit IgG at 1:10000 dilution. Predicted band size: 58 kDa. Observed band size: 58 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

100% Guaranteed
Rabbit Polyclonal to Human ALPI / Alkaline Phosphatase
Human
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human ALPI / Alkaline Phosphatase
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin
  • Immunofluorescence (1:100)
  • Western blot (1:1000)

Specificity and Use

LS-E3606 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C98228. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa274-304
This Alkaline Phosphatase (ALPI) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-304 amino acids from the Central region of human Alkaline Phosphatase (ALPI).

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About ALPI / Alkaline Phosphatase

P09923 NM_001631 NP_001622.2

ALPI Antibody, Alkaline phosphomonoesterase Antibody, Kasahara isozyme Antibody, Glycerophosphatase Antibody, Alkaline Phosphatase Antibody, IAP Antibody

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.

Immunohistochemistry

Immunohistochemical of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical of paraffin-embedded H. small intestine section using Alkaline Phosphatase (ALPI) Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunofluorescence

Fluorescent confocal image of HeLa cells stained with Alkaline Phosphatase (ALPI) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Alkaline Phosphatase (ALPI) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Alkaline Phosphatase (ALPI) immunoreactivity is localized to the cytoplasm of HeLa cells.
Fluorescent confocal image of HeLa cells stained with Alkaline Phosphatase (ALPI) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Alkaline Phosphatase (ALPI) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Alkaline Phosphatase (ALPI) immunoreactivity is localized to the cytoplasm of HeLa cells.

Western blot

Western blot of lysate from human placenta tissue lysate, using ALPI Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of lysate from human placenta tissue lysate, using ALPI Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Western blot

Western blot of lysate from human placenta tissue,using ALPI Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
Western blot of lysate from human placenta tissue,using ALPI Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.

Requested From: 
Date Requested: 5/20/2018

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