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Anti-Alpha-Fetoprotein Antibody (clone 6E6) IHC-plus™ LS-B3881

Note: This antibody replaces LS-C108761

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Alpha-Fetoprotein Antibodies

Anti-Alpha-Fetoprotein Antibody (aa19-605) LS-C123534
Goat Polyclonal (IgG) to Mouse Alpha-Fetoprotein
Mouse
Western blot, ELISA
Unconjugated
Anti-Alpha-Fetoprotein Antibody (HRP) LS-C129014
Mouse Monoclonal (IgG2b,k) to Human Alpha-Fetoprotein
Human
ELISA
HRP Conjugated
Anti-Alpha-Fetoprotein Antibody LS-C315775
Goat Polyclonal to Human Alpha-Fetoprotein
Human
IHC, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Immunodiffusion
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 6E6] (IgG2b) to Human Alpha-Fetoprotein
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human Alpha-Fetoprotein
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [6E6]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (1:200 - 1:400)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000)

Specificity and Use

Alpha-Fetoprotein antibody was raised against purified recombinant fragment of human AFP expressed in E. coli.
Human AFP
Immunohistochemistry: LS-B3881 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3881 was determined to be 1:200.

Packaging

Liquid in PBS, 50% glycerol, 0.03% sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Alpha-Fetoprotein

P02771 NM_001134 NP_001125.1

AFP Antibody, Alpha-1-fetoprotein Antibody, Alpha-fetoprotein Antibody, HPAFP Antibody, Alpha-fetoglobulin Antibody, FETA Antibody

AFP / Alpha Fetoprotein is alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology.

Immunohistochemistry

Immunohistochemistry
Anti-Alpha-Fetoprotein antibody IHC of human liver. human. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3881 dilution 1:200.

Western blot

Western blot
Western blot using AFP mouse monoclonal antibody against HepG2 (1) and SMMC-7721 (2) cell lysate.

Requested From: United States
Date Requested: 12/7/2016

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