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| Catalog Number | Size | Price |
|---|---|---|
| LS-C59288-2 | 2 ml (75 mg/ml) | $1,225 |
Polyclonal Rabbit anti‑E. coli Alkaline Phosphatase Antibody (WB) LS‑C59288
Polyclonal Rabbit anti‑E. coli Alkaline Phosphatase Antibody (WB) LS‑C59288
Antibody:
Alkaline Phosphatase Rabbit anti-E. coli Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
E. coli
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Alkaline Phosphatase Rabbit anti-E. coli Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
E. coli
Format:
Unconjugated, Unmodified
Specifications
Description
Alkaline Phosphatase antibody LS-C59288 is an unconjugated rabbit polyclonal antibody to e. coli Alkaline Phosphatase. Validated for ELISA and WB. Cited in 3 publications.
Host
Rabbit
Reactivity
E. coli
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
Alkaline Phosphatase [E.coli]
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Alkaline Phosphatase [E.coli]. No cross reactivity occurs against the mammalian form of Alkaline Phosphatase.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:8000 - 1:40000)
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of Alkaline Phosphatase [E. coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884 and ABTS as a substrate for 30 minutes at room temperature.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 2 mL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
LSBio Ratings
Alkaline Phosphatase Antibody for WB/Western, ELISA LS-C59288 has an LSBio Rating of
Publications (4.2)
Learn more about The LSBio Ratings Algorithm
Publications (3)
The clinical use of alkaline phosphatase enzymes. Epstein E, Kiechle FL, Artiss JD, Zak B. Clinics in laboratory medicine. 1986 6:491-505.
Placental alkaline phosphatase as a tumor marker for seminoma. Lange PH, Millan JL, Stigbrand T, Vessella RL, Ruoslahti E, Fishman WH. Cancer research. 1982 42:3244-7.
Recombinant Antibodies with Unique Specificities Allow for Sensitive and Specific Detection of Uncarboxylated Osteocalcin in Human Circulation. Milja Arponen, Eeva-Christine Brockmann, Riku Kiviranta, Urpo Lamminmäki, Kaisa K Ivaska. Calcified tissue international. 2020 December;107:529-542.
Customer Reviews (0)
Featured Products
Species:
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
Immunoprecipitation, ELISA, Radioimmunoassay
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence
Reactivity:
Human
Range:
5-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
