LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-ALCAM / CD166 Antibody (aa28-527, Biotin) LS-C125325

Ordering

Wt. Vol. Conc. Price
250 µg - - $725
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ALCAM / CD166 Antibodies

Anti-ALCAM / CD166 Antibody (clone 3A6, FITC) LS-C134791
Mouse Monoclonal [clone 3A6] (IgG1) to Human ALCAM / CD166
Human
Flow Cytometry, Functional Assay
FITC Conjugated
Flow Cytometry Image
Anti-ALCAM / CD166 Antibody LS-C150147
Goat Polyclonal (IgG) to Mouse ALCAM / CD166
Mouse
IHC, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human ALCAM / CD166
Human
Western blot, Flow Cytometry
Biotin Conjugated

Details

Human ALCAM / CD166
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 - 2 µg/ml)
  • Flow Cytometry (25 µg/ml)

Specificity and Use

ALCAM / CD166 antibody was raised against purified, NS0-derived, recombinant human ALCAM extracellular domain (rhALCAM; aa 28-527; Accession No. Q13740).
aa28-527
Detects Recombinant, Human ALCAM in Western blots. No cross-reactivity seen with Recombinant, Murine ALCAM, rhBCAM, rhTROP-2, rhEpCAM, rhMCAM, rhNCAML1, rmMadCAM or rmOCAM.
Suitable for use in Western Blot, Flow Cytometry. Western Blot: 1-2 ug/ml. Using a colorimetric detection system, the detection limit for rhALCAM is approximately 5 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow cytometry: 25 ug/ml and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Sterile-filtered, lyophilized powder in PBS, pH 7.2, BSA
Sterile PBS
Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C.
For research use only.

About ALCAM / CD166

Q13740 NM_001627 NP_001618.2

ALCAM Antibody, CD166 antigen Antibody, MEMD Antibody, CD166 Antibody

Activated leukocyte cell adhesion molecule (ALCAM)/cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. Homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation.

Requested From: United States
Date Requested: 12/7/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number