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Anti-AKT3 Antibody (aa119-131) LS-C3627

Note: This antibody replaces LS-C3621


Wt. Vol. Conc. Price
- 100 µl - $560
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular AKT3 Antibodies

Anti-AKT3 Antibody (N-Terminus) IHC-plus™ LS-B1042
Rabbit Polyclonal to Human AKT3
Human, Mouse
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-AKT3 Antibody (clone 25F6.F6.D8) LS-C153788
Mouse Monoclonal [clone 25F6.F6.D8] (IgG1) to Human AKT3
Western blot, ELISA
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human AKT3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
Western blot, Immunoprecipitation, Functional Assay


Human AKT3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Hamster, Zebrafish (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
  • Functional Assay

Specificity and Use

AKT3 antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa119-131 (CSPTSQIDNIGEE) of human Akt3/PKBg. The immunizing sequence is identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Hamster, Zebrafish (92%).
Recognizes Akt3/PKBg, Mr 60kD. An additional protein was observed, Mr 50kD. Detection of both bands is inhibited by preincubation with the immunizing peptide. Species cross-reactivity: Human. Species sequence homology: mouse and rat.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: A 1:500-1:2000 dilution of this lot detected Akt3/PKBg in HEK293 cell lysates. Immunoprecipitation: 5 ul immunoprecipitated active Akt3/PKBg from 15 nM IGF-I stimulated HEK293 cell lysates. The kinase activity of immunoprecipitated Akt3/PKBg was determined using Akt/SGK Specific Substrate Peptide Active kinase was detected by comparing the incorporation of [32P] into Akt/SGK Substrate Peptide as catalyzed by immunoprecipitates from IGF-I stimulated (15nM) HEK293 cell lysates (1mg).


0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About AKT3

Q9Y243 NM_005465 NP_005456.1

AKT3 Antibody, AKT3/PKBgamma Antibody, PRKBG Antibody, PKB gamma Antibody, Protein kinase Akt-3 Antibody, RAC-gamma Antibody, MPPH Antibody, PKB-GAMMA Antibody, PKBG Antibody, Protein kinase B gamma Antibody, RAC-PK-gamma Antibody, STK-2 Antibody

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform.

Requested From: United States
Date Requested: 10/23/2016

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