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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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AKT3 Antibody (aa119‑131) LS‑C3627
Note: This antibody replaces LS-C3621
AKT3 antibody LS-C3627 is an unconjugated rabbit polyclonal antibody to AKT3 from human, mouse, rat and other species. Validated for Func, IP and WB.
Antibody
Catalog
Size
Price
LS-C3627-100
100 µl
$635

Popular AKT3 Products

Anti-AKT3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1042 concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-AKT3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1319 concentration 10 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Anti-AKT3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3935 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-AKT3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4825 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Western blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 antibody at 1:1000 for overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.
Species: Human
Applications: Western blot, ELISA

Product Description

AKT3 antibody LS-C3627 is an unconjugated rabbit polyclonal antibody to AKT3 from human, mouse, rat and other species. Validated for Func, IP and WB.
About AKT3
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. Q9Y243 NM_005465 NP_005456.1

AKT3 Antibody, AKT3/PKBgamma Antibody, PRKBG Antibody, PKB gamma Antibody, Protein kinase Akt-3 Antibody, RAC-gamma Antibody, MPPH Antibody, PKB-GAMMA Antibody, PKBG Antibody, Protein kinase B gamma Antibody, RAC-PK-gamma Antibody, STK-2 Antibody


Specifications

Target
Human AKT3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Predicted
Hamster, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
  • Functional Assay
  • (applications tested for the base form of this product only)
Immunogen
AKT3 antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa119-131 (CSPTSQIDNIGEE) of human Akt3/PKBg. The immunizing sequence is identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Hamster, Zebrafish (92%).
Epitope
aa119-131
Specificity
Recognizes Akt3/PKBg, Mr 60kD. An additional protein was observed, Mr 50kD. Detection of both bands is inhibited by preincubation with the immunizing peptide. Species cross-reactivity: Human. Species sequence homology: mouse and rat.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: A 1:500-1:2000 dilution of this lot detected Akt3/PKBg in HEK293 cell lysates. Immunoprecipitation: 5 ul immunoprecipitated active Akt3/PKBg from 15 nM IGF-I stimulated HEK293 cell lysates. The kinase activity of immunoprecipitated Akt3/PKBg was determined using Akt/SGK Specific Substrate Peptide Active kinase was detected by comparing the incorporation of [32P] into Akt/SGK Substrate Peptide as catalyzed by immunoprecipitates from IGF-I stimulated (15nM) HEK293 cell lysates (1mg).
Presentation
0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/19/2018

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