AKT3 antibody LS-C3627 is an unconjugated rabbit polyclonal antibody to AKT3 from human, mouse, rat and other species. Validated for Func, IP and WB.
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
(tested or 100% immunogen sequence identity)
Hamster, Zebrafish (at least 90% immunogen sequence identity)
Also available conjugated with AP
- Western blot (1:500 - 1:2000)
- Functional Assay
- (applications tested for the base form of this product only)
AKT3 antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa119-131 (CSPTSQIDNIGEE) of human Akt3/PKBg. The immunizing sequence is identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Hamster, Zebrafish (92%).
Recognizes Akt3/PKBg, Mr 60kD. An additional protein was observed, Mr 50kD. Detection of both bands is inhibited by preincubation with the immunizing peptide. Species cross-reactivity: Human. Species sequence homology: mouse and rat.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: A 1:500-1:2000 dilution of this lot detected Akt3/PKBg in HEK293 cell lysates. Immunoprecipitation: 5 ul immunoprecipitated active Akt3/PKBg from 15 nM IGF-I stimulated HEK293 cell lysates. The kinase activity of immunoprecipitated Akt3/PKBg was determined using Akt/SGK Specific Substrate Peptide Active kinase was detected by comparing the incorporation of [32P] into Akt/SGK Substrate Peptide as catalyzed by immunoprecipitates from IGF-I stimulated (15nM) HEK293 cell lysates (1mg).
0.2 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.1 mM EDTA, 0.05% sodium azide, before the addition of glycerol to 30%.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.