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Anti-AKT2 Antibody (clone 16G11.A7) LS-C153804

Catalog Size Price
LS-C153804-100 100 µg Unavailable

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100% Guaranteed
Rat Monoclonal [clone 16G11.A7] (IgG2a) to Human AKT2
Human
Western blot, ELISA
Unconjugated

Details

Human AKT2
Rat
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [16G11.A7]
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot (1:500 - 1:2000)
  • ELISA

Specificity and Use

AKT2 antibody was raised against anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.
Anti-AKT2 Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About AKT2

P31751 NM_001626 NP_001617.1

AKT2 Antibody, PKB beta Antibody, PKBBETA Antibody, PRKBB Antibody, Protein kinase Akt-2 Antibody, Protein kinase B beta Antibody, RAC-PK-beta Antibody, Rac protein kinase beta Antibody, RAC-BETA Antibody, HIHGHH Antibody, PKBB Antibody

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Western blot

Western blot
Western blot of Rat anti-AKT2 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4C. Secondary antibody: Peroxidase rat secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none.

Requested From: 
Date Requested: 10/21/2017

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