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Catalog Number Size Price
LS-C209266-100 100 µg (1 mg/ml) $574 
AKT2 Antibody - Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: Goat secondary antibody anti rat at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 85 kDa for GST-AKT2.

Monoclonal Rat anti‑Human AKT2 Antibody (clone 11F6.B2, Internal, IHC, WB) LS‑C209266

Monoclonal Rat anti‑Human AKT2 Antibody (clone 11F6.B2, Internal, IHC, WB) LS‑C209266

Antibody:
AKT2 Rat anti-Human Monoclonal (Internal) (11F6.B2) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$574
LS-C209266-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
AKT2 Rat anti-Human Monoclonal (Internal) (11F6.B2) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AKT2 antibody LS-C209266 is an unconjugated rat monoclonal antibody to human AKT2 (Internal). Validated for ELISA, Flow, IHC and WB.
Target
Human AKT2
Synonyms
AKT2 | PKB beta | PKBBETA | PRKBB | Protein kinase Akt-2 | Protein kinase B beta | RAC-PK-beta | Rac protein kinase beta | RAC-BETA | HIHGHH | PKBB
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
11F6.B2
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Epitope
Internal
Applications
  • IHC (20 µg/ml)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-AKT2 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT2
P31751 NM_001626 NP_001617.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024