Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
AKT2 Antibody, PKB beta Antibody, PKBBETA Antibody, PRKBB Antibody, Protein kinase Akt-2 Antibody, Protein kinase B beta Antibody, RAC-PK-beta Antibody, Rac protein kinase beta Antibody, RAC-BETA Antibody, HIHGHH Antibody, PKBB Antibody
AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with AKT2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of AKT2 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot of anti-AKT2 Antibody antibody in mouse liver tissue lysates (35 ug/lane). AKT2(arrow) was detected using the purified antibody.
Western blot of anti-AKT2 Antibody antibody in 293 cell line lysates transiently transfected with the AKT2 gene (2 ug/lane). AKT2(arrow) was detected using the purified antibody.
AKT2 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.