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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2541-50 | 50 µg (0.5 mg/ml) | $485 |
IHC‑plus™ Monoclonal Mouse anti‑Human AKT1S1 / PRAS40 Antibody (IHC, WB) LS‑B2541
IHC‑plus™ Monoclonal Mouse anti‑Human AKT1S1 / PRAS40 Antibody (IHC, WB) LS‑B2541
Note: This antibody replaces LS-C17345
Antibody:
AKT1S1 / PRAS40 Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
AKT1S1 / PRAS40 Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
PRAS40 antibody LS-B2541 is an unconjugated mouse monoclonal antibody to human PRAS40 (AKT1S1). Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human AKT1S1 / PRAS40
Synonyms
AKT1S1 | PRAS40 | Proline-rich AKT1 substrate 1 | Lobe
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Immunogen
Recombinant full length human PRAS40 expressed in E. coli.
Specificity
Recognizes human PRAS40. Reacts with a 40kD band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not cross-react with mouse.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (0.1 - 1 µg/ml)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2541 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2541 was determined to be 10 ug/ml. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 ug/ml. Positive control: HeLa cells; Jurkat cells.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide, 1% BSA
Storage
Short term: 4°C; Long term: -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About AKT1S1 / PRAS40
Validation
Anti-AKT1S1 / PRAS40 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-AKT1S1 / PRAS40 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ AKT1S1 / PRAS40 Antibody for IHC, WB/Western, IP LS-B2541 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Human, Monkey, Bat
Applications:
IHC - Paraffin
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Reactivity:
All species
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
Date Requested: 4/17/2024
