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Anti-AKT1S1 / PRAS40 Antibody IHC-plus™ LS-B2541

Note: This antibody replaces LS-C17345

Ordering

Wt. Vol. Conc. Price
50 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AKT1S1 / PRAS40 Antibodies

Anti-AKT1S1 / PRAS40 Antibody (aa258-276) LS-C194027
Rabbit Polyclonal (IgG) to Human AKT1S1 / PRAS40
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
Western blot, Immunoprecipitation
Unconjugated
Anti-AKT1S1 / PRAS40 Antibody LS-C194028
Rabbit Polyclonal (IgG) to Human AKT1S1 / PRAS40
Human
Western blot
Unconjugated
Anti-AKT1S1 / PRAS40 Antibody (clone 449CT13.4.2, AP) LS-C206306
Mouse Monoclonal [clone 449CT13.4.2] (IgG1) to Human AKT1S1 / PRAS40
Human
Western blot, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1,k) to Human AKT1S1 / PRAS40
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human AKT1S1 / PRAS40
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated
Protein A/G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.1 - 1 µg/ml)
  • Immunoprecipitation

Specificity and Use

AKT1S1 / PRAS40 antibody was raised against recombinant full length human PRAS40 expressed in E. coli.
Recognizes human PRAS40. Reacts with a 40kD band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not cross-react with mouse.
Immunohistochemistry: LS-B2541 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2541 was determined to be 10 ug/ml. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 ug/ml. Positive control: HeLa cells; Jurkat cells.

Packaging

PBS, pH 7.2, 0.1% sodium azide, 1% BSA.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About AKT1S1 / PRAS40

Q96B36 NM_032375 NP_115751.3

AKT1S1 Antibody, PRAS40 Antibody, Proline-rich AKT1 substrate 1 Antibody, Lobe Antibody

Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1.

Immunohistochemistry

Immunohistochemistry
Anti-AKT1S1 / PRAS40 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2541 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/5/2016

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