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Anti-AKT1 Antibody (phospho-Ser473, clone 17F6.B11, Biotin) LS-C153789

Ordering

Wt. Vol. Conc. Price
50 µg 100 µl 0.5 mg/ml $345
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular AKT1 Antibodies

Anti-AKT1 Antibody (phospho-Ser473) IHC-plus™ LS-B1183
Rabbit Polyclonal to Human AKT1
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-AKT1 Antibody (aa121-170) IHC-plus™ LS-B1510
Rabbit Polyclonal (IgG) to Human AKT1
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Sheep, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AKT1 Antibody (phospho-Thr450, PE) LS-C252845
Rabbit Polyclonal (IgG) to Human AKT1
Human
ELISA, Dot Blot
PE Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 17F6.B11] (IgG) to Human AKT1
Human, Monkey, Mouse, Rat
IHC, Immunofluorescence, Western blot, ELISA
Biotin Conjugated

Details

Human AKT1
Mouse
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Monoclonal [17F6.B11]
Biotin
Protein A purified
Unmodified

Applications

  • IHC (20 µg/ml)
  • Immunofluorescence (1:500 - 1:3000)
  • Western blot (1:500 - 1:3000)
  • ELISA

Specificity and Use

AKT1 antibody was raised against anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
pSer473
This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
Biotin Conjugated Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kD in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% sodium azide
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About AKT1

P31749 NM_005163 NP_005154.2

AKT1 Antibody, AKT Antibody, AKT-1 Antibody, C-AKT Antibody, Pan-AKT Antibody, PKB alpha Antibody, PKBalpha Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, Rac protein kinase alpha Antibody, PRKBA Antibody, Protein kinase B alpha Antibody, RAC-PK-alpha Antibody, PKB Antibody, PKB-ALPHA Antibody, RAC Antibody, RAC-ALPHA Antibody

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Immunohistochemistry

Immunohistochemistry
Immunohistochemistry of mouse anti AKT phospho S473 biotin conjugated at 40X (left) with negative control (right) Tissue: prostate Fixation: FFPE buffered formalin 10% conc Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 20 g/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: none. Load: 25 ng per lane. Primary antibody: Akt phospho S473 Biotin Conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho S473. Other band(s): none This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/7/2016

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