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Anti-AKT1 Antibody (Internal) LS-C60161

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AKT1 Antibodies

Anti-AKT1 Antibody (phospho-Ser473) IHC-plus™ LS-B1183
Rabbit Polyclonal to Human AKT1
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-AKT1 Antibody (aa121-170) IHC-plus™ LS-B1510
Rabbit Polyclonal (IgG) to Human AKT1
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Sheep, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AKT1 Antibody (phospho-Thr450, PE) LS-C252845
Rabbit Polyclonal (IgG) to Human AKT1
Human
ELISA, Dot Blot
PE Conjugated

100% Guaranteed 100% Guaranteed
Sheep Polyclonal to Human AKT1
Human
Western blot, ELISA
Unconjugated

Details

Human AKT1
Sheep
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Unmodified

Applications

  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:20000)

Specificity and Use

AKT1 antibody was raised against synthetic peptide corresponding to an internal region of human AKT1 protein. A residue of cysteine was added to facilitate coupling.
Internal
AKT and reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse and chicken AKT
This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 55-60 kD in size corresponding to AKT1 by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About AKT1

P31749 NM_005163 NP_005154.2

AKT1 Antibody, AKT Antibody, AKT-1 Antibody, C-AKT Antibody, Pan-AKT Antibody, PKB alpha Antibody, PKBalpha Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, Rac protein kinase alpha Antibody, PRKBA Antibody, Protein kinase B alpha Antibody, RAC-PK-alpha Antibody, PKB Antibody, PKB-ALPHA Antibody, RAC Antibody, RAC-ALPHA Antibody

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Western blot

Western blot
Western Blot - Anti-AKT Antibody. Anti-AKT Antibody in western blot showing detection of endogenous protein in whole cell extracts from H2O2 treated MCF7 cells (lane 2). Recombinant AKT is detected in lane 3. Lanes 1 and 4 contain wcl from HeLa and A431 cells, respectively. Specific band staining is completely blocked when antibody is pre-incubated with the immunizing peptide (data not shown). The band at ~55-60 kD, indicated by the arrowhead, corresponds to the expected molecular weight of AKT. AKT antibody was diluted 1:300 in 1% BLOTTO and reacted with the membrane overnight at 4° C. IRDYE800 conjugated Dnky-a-Sheep IgG was used at a 1:10000 dilution for 45 min at room temperature.
 

Requested From: United States
Date Requested: 12/10/2016

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