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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AKT1 Antibody (clone 4D6) LS‑C173708
AKT1 antibody LS-C173708 is an unconjugated mouse monoclonal antibody to AKT1 from human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C173708-100
100 µl (0.73 mg/ml)
Unavailable

Popular AKT1 Products

Immunohistochemistry of Rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Anti-AKT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1510 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Sheep, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: ELISA, Dot Blot

Product Description

AKT1 antibody LS-C173708 is an unconjugated mouse monoclonal antibody to AKT1 from human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
About AKT1
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. P31749 NM_005163 NP_005154.2

AKT1 Antibody, AKT Antibody, AKT-1 Antibody, C-AKT Antibody, Pan-AKT Antibody, PKB alpha Antibody, PKBalpha Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, Rac protein kinase alpha Antibody, PRKBA Antibody, Protein kinase B alpha Antibody, RAC-PK-alpha Antibody, PKB Antibody, PKB-ALPHA Antibody, RAC Antibody, RAC-ALPHA Antibody


Specifications

Target
Human AKT1
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [4D6]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Immunofluorescence (1:100)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
AKT1 antibody was raised against full length human recombinant protein of human AKT1(NP_005154) produced in HEK293T cell.
Specificity
Human AKT1
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.

Immunofluorescence

Anti-AKT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.
Anti-AKT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-AKT1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-AKT1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.

Immunofluorescence

Anti-AKT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.
Anti-AKT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-AKT1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-AKT1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.

Immunofluorescence

Anti-AKT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.
Anti-AKT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-AKT1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-AKT1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human prostate tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human prostate tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 mouse monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.
IHC of paraffin-embedded Human Kidney tissue using anti-AKT1 mouse monoclonal antibody.

Immunofluorescence

Anti-AKT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.
Anti-AKT1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-AKT1 monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-AKT1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-AKT1 antibody (Red), compared to a nonspecific negative control antibody (Blue).


Requested From: 
Date Requested: 6/24/2018

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