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Anti-AKT1 Antibody (phospho-Ser473) IHC-plus™ LS-B1183

Note: This antibody replaces LS-C18880


Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)

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Rabbit Polyclonal to Human AKT1
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA


Human AKT1
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Immunoaffinity purified


IHC - Paraffin (5 µg/ml), ICC, Immunofluorescence, Western blot (1:200 - 1:1000), ELISA (1:15000 - 1:60000)

Specificity and Use

AKT1 antibody was raised against synthetic peptide from human AKT1.
The C-terminus aa 460-480 of human, mouse, rat and chicken AKT proteins conjugated to KLH.
Immunohistochemistry: LS-B1183 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1183 was determined to be 5 ug/ml.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About AKT1

P31749 NM_005163 NP_005154.2

AKT1 Antibody, AKT Antibody, AKT-1 Antibody, C-AKT Antibody, Pan-AKT Antibody, PKB alpha Antibody, PKBalpha Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, Rac protein kinase alpha Antibody, PRKBA Antibody, Protein kinase B alpha Antibody, RAC-PK-alpha Antibody, PKB Antibody, PKB-ALPHA Antibody, RAC Antibody, RAC-ALPHA Antibody

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.


Immunohistochemistry of Rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.


Anti-AKT1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1183 concentration 5 ug/ml.


Anti-AKT1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1183 concentration 5 ug/ml.


Anti-AKT pS473 Antibody - Immunofluorescence Microscopy. Confocal image using Rabbit anti-AKT pS473 at a 1:40 dilution to detect phosphorylated AKT (green) by immunofluorescence microscopy in cardiomyocytes infected with adenovirus expressing with wild-type AKT in conjunction with a Texas-red conjugated phalloidin (red) to label filamentous actin.

Western blot

Western blot
Anti-AKT pS473 Antibody - Western Blot. Rabbit anti-AKT pS473 was used at a 1:200 dilution to detect phosphorylated AKT by Western blot. A nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase was used.

Requested From: United States
Date Requested: 11/28/2015

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