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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AKT1 Antibody (aa49‑76) LS‑C156226
AKT1 antibody LS-C156226 is an unconjugated rabbit polyclonal antibody to human AKT1. Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C156226-400
400 µl
Unavailable

Popular AKT1 Products

Immunohistochemistry of Rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Anti-AKT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1510 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Sheep, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: ELISA, Dot Blot

Product Description

AKT1 antibody LS-C156226 is an unconjugated rabbit polyclonal antibody to human AKT1. Validated for Flow, IF and WB.
About AKT1
AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. P31749 NM_005163 NP_005154.2

AKT1 Antibody, AKT Antibody, AKT-1 Antibody, C-AKT Antibody, Pan-AKT Antibody, PKB alpha Antibody, PKBalpha Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, Rac protein kinase alpha Antibody, PRKBA Antibody, Protein kinase B alpha Antibody, RAC-PK-alpha Antibody, PKB Antibody, PKB-ALPHA Antibody, RAC Antibody, RAC-ALPHA Antibody


Specifications

Target
Human AKT1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Blocking Peptide
LS-E8714 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156226. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa49-76
Specificity
This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-76 amino acids from the N-terminal region of human AKT1.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Confocal immunofluorescence of AKT1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of AKT1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of AKT1 Antibody in MCF-7 cell line lysates (35 ug/lane). AKT1 (arrow) was detected using the purified antibody.
Western blot of AKT1 Antibody in MCF-7 cell line lysates (35 ug/lane). AKT1 (arrow) was detected using the purified antibody.

Flow Cytometry

AKT1 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AKT1 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence

Confocal immunofluorescence of AKT1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of AKT1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of AKT1 Antibody in MCF-7 cell line lysates (35 ug/lane). AKT1 (arrow) was detected using the purified antibody.
Western blot of AKT1 Antibody in MCF-7 cell line lysates (35 ug/lane). AKT1 (arrow) was detected using the purified antibody.

Flow Cytometry

AKT1 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AKT1 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence

Confocal immunofluorescence of AKT1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of AKT1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of AKT1 Antibody in MCF-7 cell line lysates (35 ug/lane). AKT1 (arrow) was detected using the purified antibody.
Western blot of AKT1 Antibody in MCF-7 cell line lysates (35 ug/lane). AKT1 (arrow) was detected using the purified antibody.

Flow Cytometry

AKT1 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AKT1 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence

Confocal immunofluorescence of AKT1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of AKT1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of AKT1 Antibody in MCF-7 cell line lysates (35 ug/lane). AKT1 (arrow) was detected using the purified antibody.
Western blot of AKT1 Antibody in MCF-7 cell line lysates (35 ug/lane). AKT1 (arrow) was detected using the purified antibody.

Flow Cytometry

AKT1 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AKT1 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/20/2018

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