Human AKT1

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

Sf 21-derived recombinant human v-akt murine thymoma viral oncogene homolog 1 aa2-480.

aa2-480

Recognizes human Akt1. Does not detect rhAkt2 or rhAkt3.

• ICC (8 - 25 µg/ml)
• Western blot
• Flow Cytometry (50 µg/ml)
• ELISA

Suitable for use in ELISA, Western Blot, Immunocytochemistry, Suitable for use in Flow cytometry: 50 ug/ml. This antibody was validated for flow cytometry using MCF-7 cells. For. intracellular staining to detect Akt1, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 50 ug/ml and add 10 ul of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 20ulL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Immunocytochemistry: 8-25 ug/ml to detect Akt1 in fixed human peripheral blood mononuclear cells.

Lyophilized from Sterile Solution PBS, 5% Trehalose

Reconstitute in sterile PBS.

Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P31749 NCBI: NM_005163 NP_005154.2