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Anti-AKT1 Antibody (aa115-144) LS-C98346

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AKT1 Antibodies

Anti-AKT1 Antibody (phospho-Ser473) IHC-plus™ LS-B1183
Rabbit Polyclonal to Human AKT1
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-AKT1 Antibody (aa121-170) IHC-plus™ LS-B1510
Rabbit Polyclonal (IgG) to Human AKT1
Human, Monkey, Mouse, Rat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Sheep, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AKT1 Antibody (phospho-Thr450, PE) LS-C252845
Rabbit Polyclonal (IgG) to Human AKT1
Human
ELISA, Dot Blot
PE Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human AKT1
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human AKT1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

AKT1 antibody was raised against kLH-conjugated synthetic peptide between amino acids 115-144 from N-terminal region of human AKT1.
LS-E8713 - Lyophilized - 100 µg - $145.00
aa115-144

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About AKT1

P31749 NM_005163 NP_005154.2

AKT1 Antibody, AKT Antibody, AKT-1 Antibody, C-AKT Antibody, Pan-AKT Antibody, PKB alpha Antibody, PKBalpha Antibody, Proto-oncogene c-Akt Antibody, Protein kinase B Antibody, Rac protein kinase alpha Antibody, PRKBA Antibody, Protein kinase B alpha Antibody, RAC-PK-alpha Antibody, PKB Antibody, PKB-ALPHA Antibody, RAC Antibody, RAC-ALPHA Antibody

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with AKT1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of AKT1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot
AKT1 Antibody western blot of HeLa cell line lysates (35 ug/lane). The AKT1 antibody detected the AKT1 protein (arrow).

Western blot

Western blot
Western blot of AKT1 Antibody polyclonal antibody(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (Lane 2) (Origene Technologies).

Flow Cytometry

Flow Cytometry
AKT1 Antibody flow cytometry of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

Requested From: United States
Date Requested: 12/3/2016

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