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Anti-AKT1 + AKT2 + AKT3 Antibody (aa313-317) LS-C205390

Catalog	Size	Price
LS-C205390-50	50 µg (1 mg/ml)	Unavailable

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Western blot of extracts from HeLa cells treated with heat shock, using Akt (Phospho-Ser473) Antibody. The lane on the left is treated with the synthesized peptide.

Anti-AKT1 + AKT2 + AKT3 Antibody (Internal) LS-C351801

Antibody:	Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3
Reactivity:	Human, Mouse, Rat, Bovine, Dog, Chicken, Zebrafish
Application:	IHC, IHC - Paraffin, Western blot
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Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

Anti-AKT1 + AKT2 + AKT3 Antibody (Internal) LS-C354521

Antibody:	Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3
Reactivity:	Human, Mouse, Rat, Bovine, Sheep
Application:	IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
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Immunohistochemical analysis of AKT staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w

Anti-AKT1 + AKT2 + AKT3 Antibody (C-Terminus) LS-C354963

Antibody:	Rabbit Polyclonal (IgG) to Human AKT1 + AKT2 + AKT3
Reactivity:	Human, Mouse
Application:	ICC, Immunofluorescence, Western blot, Flow Cytometry
Format:	Unconjugated

Antibody:	Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3
Reactivity:	Human, Mouse, Rat
Application:	IHC, IHC - Paraffin
Format:	Unconjugated

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (aa313-317/316/312) antibody (left) or the same antibody preincubated with blocking peptide (right).

Details

Target:	Human AKT1 + AKT2 + AKT3
Host:	Rabbit
Reactivity:	Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

IHC
IHC - Paraffin (1:50 - 1:100)

Specificity and Use

Immunogen:	Peptide sequence around amino acids 313-317/314-318/310-314 (P-E-Y-L-A) of human AKT1/AKT2/AKT3.
Epitope:	aa313-317
Specificity:	The antibody detects endogenous levels of total AKT1/AKT2/AKT3 protein.

Packaging

Presentation:	PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Storage:	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About AKT1 + AKT2 + AKT3

Description: AKT, also known as protein kinase B (PKB), constitutes a family of serine/threonine kinases. AKT has 3 isoforms: AKT1, AKT2), and AKT3). These isoforms share a high degree of homology, especially in the catalytic domains (87%-90% identical amino acids).

(More About AKT1 + AKT2 + AKT3)

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Immunohistochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (aa313-317/316/312) antibody (left) or the same antibody preincubated with blocking peptide (right).

Requested From:
Date Requested: 4/21/2018

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