Human AGRP

Goat

Human, Monkey, Bat, Horse (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Unmodified

Peptide with sequence CRKLGTAMNPCSRT, from the C-Terminus of protein sequence according to NP_001129.1NP_015531.1.

aa119-132

Human AGRP. This antibody is expected to recognise both reported isoforms (as represented by NP_001129; NP_015531)

• IHC - Frozen (0.05 - 0.1 µg/ml)
• Peptide Enzyme-Linked Immunosorbent Assay (1:32000)

Preliminary experiments gave bands at approx 45kDa and 24kDa in Human Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.4kDa according to NP_001129.1and NP_015531.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O00253 NCBI: NM_001138 NP_001129.1