Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone IMG10E2] (IgG1,k) to Human AGR2
Human, Mouse, Bovine, Dog, Horse
IHC, IHC - Paraffin, Western blot
Rabbit Polyclonal to Human AGR2
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Human (tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
IHC - Paraffin (1:10 - 1:50)
Immunofluorescence (1:10 - 1:50)
Western blot (1:1000)
Specificity and Use
LS-E8174 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99989. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
This Anterior Gradient 2 (AGR2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Anterior Gradient 2 (AGR2).
PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells By similarity. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent confocal image of A549 cell stained with AGR2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
AGR2 Antibody western blot of HT29,MCF-7,ZR-75-1 cell line lysates (35 ug/lane). The AGR2 antibody detected the AGR2 protein (arrow).