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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AGR2 Antibody (aa13‑42) LS‑C99989
AGR2 antibody LS-C99989 is an unconjugated rabbit polyclonal antibody to human AGR2. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C99989-400
400 µl
Unavailable

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Product Description

AGR2 antibody LS-C99989 is an unconjugated rabbit polyclonal antibody to human AGR2. Validated for IF, IHC and WB.
About AGR2
Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells By similarity. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. O95994 NM_006408 NP_006399.1

AGR2 Antibody, AG2 Antibody, AG-2 Antibody, HPC8 Antibody, HAG-2 Antibody, Secreted cement gland homolog Antibody, Anterior gradient homolog 2 Antibody, GOB-4 Antibody, PDIA17 Antibody, XAG-2 Antibody


Specifications

Target
Human AGR2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8174 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99989. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa13-42
Specificity
This Anterior Gradient 2 (AGR2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Anterior Gradient 2 (AGR2).
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Fluorescent confocal image of A549 cell stained with AGR2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
Fluorescent confocal image of A549 cell stained with AGR2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.

Western blot

AGR2 Antibody western blot of HT29,MCF-7,ZR-75-1 cell line lysates (35 ug/lane). The AGR2 antibody detected the AGR2 protein (arrow).
AGR2 Antibody western blot of HT29,MCF-7,ZR-75-1 cell line lysates (35 ug/lane). The AGR2 antibody detected the AGR2 protein (arrow).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Fluorescent confocal image of A549 cell stained with AGR2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
Fluorescent confocal image of A549 cell stained with AGR2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.

Western blot

AGR2 Antibody western blot of HT29,MCF-7,ZR-75-1 cell line lysates (35 ug/lane). The AGR2 antibody detected the AGR2 protein (arrow).
AGR2 Antibody western blot of HT29,MCF-7,ZR-75-1 cell line lysates (35 ug/lane). The AGR2 antibody detected the AGR2 protein (arrow).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Fluorescent confocal image of A549 cell stained with AGR2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
Fluorescent confocal image of A549 cell stained with AGR2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.

Western blot

AGR2 Antibody western blot of HT29,MCF-7,ZR-75-1 cell line lysates (35 ug/lane). The AGR2 antibody detected the AGR2 protein (arrow).
AGR2 Antibody western blot of HT29,MCF-7,ZR-75-1 cell line lysates (35 ug/lane). The AGR2 antibody detected the AGR2 protein (arrow).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Fluorescent confocal image of A549 cell stained with AGR2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
Fluorescent confocal image of A549 cell stained with AGR2 Antibody. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.

Western blot

AGR2 Antibody western blot of HT29,MCF-7,ZR-75-1 cell line lysates (35 ug/lane). The AGR2 antibody detected the AGR2 protein (arrow).
AGR2 Antibody western blot of HT29,MCF-7,ZR-75-1 cell line lysates (35 ug/lane). The AGR2 antibody detected the AGR2 protein (arrow).


Requested From: 
Date Requested: 6/21/2018

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