(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:100000)
ADRB3 antibody was raised against a 19 amino acid peptide sequence mapping at the C-terminus of the mouse B3AR (2) was used to generate anti- B3AR. The peptide was coupled with KLH (carrier protein).
The 20 AA mouse B3AR immunogenic peptide sequence is 85% homologous with rat B3AR (17/20). It has no significant homology with the human B3AR. Anti-B3AR11 antibodies are not likely to cross-react with mouse/rat B3AR. Actual cross-reactivity of antibodies in all species is not established. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure using ECL. ELISA: 1:10000-1:100000 using 50-100 ng B3AR control peptide/well.
PBS, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.