Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
Western blot (1:1000), ELISA
Specificity and Use
Adiponectin antibody was raised against recombinant human adiponectin.
Recognizes human adiponectin. Detects a band of ~30kD by Western Blot.
Suitable for use in ELISA and Western Blot. ELISA (Direct and Indirect): 1:5000-1:10000. Western Blot:1:1000 using ECL; Suggested blocking and dilution buffer is TBST with 0.05% Tween-20, 5% skim milk; Incubate 1 hour at RT.
PBS, pH 7.4. Contains no preservatives.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects.