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Anti-ADGRE5 / CD97 Antibody (aa1-512) LS-C18770

Ordering

Wt. Vol. Conc. Price
500 µg 500 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ADGRE5 / CD97 Antibodies

Anti-ADGRE5 / CD97 Antibody (N-Terminus) IHC-plus™ LS-A2644
Rabbit Polyclonal to Human ADGRE5 / CD97
Human
IHC - Paraffin, ICC
Unconjugated
Immunohistochemistry Image
Anti-ADGRE5 / CD97 Antibody (Cytoplasmic Domain) IHC-plus™ LS-A2652
Rabbit Polyclonal to Human ADGRE5 / CD97
Human, Monkey, Dog
IHC - Paraffin, ICC
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse ADGRE5 / CD97
Mouse, Human
Immunofluorescence, Western blot
Unconjugated

Details

Mouse ADGRE5 / CD97
Rabbit
Mouse, Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (1:1000 - 1:5000)
  • Western blot (1:1000 - 1:5000)

Specificity and Use

ADGRE5 / CD97 antibody was raised against recombinant mouse CD97.
aa1-512
Amino acids 1-512 (extracellular domain) of mouse CD97 protein.
This Protein A purified antibody has been tested for use in immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kD in size corresponding to isoform 1 of CD97 protein by western blotting in the appropriate cell lysate or extract.

Packaging

Lyophilized.
500 µl After adding water, will consist of 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2 with 0.01% (w/v) Sodium Azide
Store vial at 4 C prior to opening. Reconstitute with 0.1 ml deionized water (or equivalent). Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing.
For research use only.

About ADGRE5 / CD97

P48960 NM_078481 NP_510966.1

ADGRE5 Antibody, CD97 Antibody, CD97 molecule Antibody, Leukocyte antigen CD97 Antibody, TM7LN1 Antibody, CD97 antigen Antibody

ADGRE5 / CD97 is a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex.

Western blot

Western blot
Anti-CD97 Antibody - Western Blot. Western blot of Protein A purified anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). No staining was noted from bone marrow lysates taken from CD97 knockout mice. The identity of the band at ~65 kD appearing in all lanes is not known. The formation of the CD97 complex is currently under investigation. Approximately 10 ul of lysate was used in each lane. A 1:1000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
 

Requested From: United States
Date Requested: 12/4/2016

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