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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ADGRE1 / EMR1 Antibody (aa626‑886) LS‑C185444
EMR1 antibody LS-C185444 is an unconjugated rabbit polyclonal antibody to human EMR1 (ADGRE1). Validated for IHC and WB.
Catalog
Size
Price
LS-C185444-100
100 µl (1 mg/ml)
$335
Description
EMR1 antibody LS-C185444 is an unconjugated rabbit polyclonal antibody to human EMR1 (ADGRE1). Validated for IHC and WB.
Target
Human ADGRE1 / EMR1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ADGRE1 / EMR1 antibody was raised against recombinant fragment corresponding to a region within amino acids 626 and 886 of EMR1 (SwissProt Q14246).
Epitope
aa626-886
Specificity
Human EMR1 / F4/80
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
About ADGRE1 / EMR1
Q14246 NM_001974 NP_001965.3

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Images

Immunohistochemistry

IHC of paraffin-embedded PC13 xenograft using EMR1 antibody at 1:100 dilution.
IHC of paraffin-embedded PC13 xenograft using EMR1 antibody at 1:100 dilution.

Western blot

EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug K562 whole cell lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000
EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug K562 whole cell lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000

Western blot

EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000
EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000

Immunohistochemistry

IHC of paraffin-embedded PC13 xenograft using EMR1 antibody at 1:100 dilution.
IHC of paraffin-embedded PC13 xenograft using EMR1 antibody at 1:100 dilution.

Western blot

EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug K562 whole cell lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000
EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug K562 whole cell lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000

Western blot

EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000
EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000

Immunohistochemistry

IHC of paraffin-embedded PC13 xenograft using EMR1 antibody at 1:100 dilution.
IHC of paraffin-embedded PC13 xenograft using EMR1 antibody at 1:100 dilution.

Western blot

EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug K562 whole cell lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000
EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug K562 whole cell lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000

Western blot

EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000
EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000

Immunohistochemistry

IHC of paraffin-embedded PC13 xenograft using EMR1 antibody at 1:100 dilution.
IHC of paraffin-embedded PC13 xenograft using EMR1 antibody at 1:100 dilution.

Western blot

EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug K562 whole cell lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000
EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. B. 30 ug Raji whole cell lysate/extract. C. 30 ug K562 whole cell lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000

Western blot

EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000
EMR1 antibody [C2C3], C-term detects EMR1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract. 7.5 % SDS-PAGE. EMR1 antibody [C2C3], C-term dilution:1:1000

Requested From: United States
Date Requested: 12/15/2018
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