(tested or 100% immunogen sequence identity)
Also available conjugated with APC
- Flow Cytometry
- (applications tested for the base form of this product only)
ADAM10 antibody was raised against mouse myeloma cell line NS0-derived recombinant human ADAM10 corresponding to aa 214-672 (UniProt Accession # O14672).
Recognizes the ectodomain of human ADAM10.
Suitable for use in Flow cytometry: Intracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells. To stain for surface proteins (e. g. CD3, CD4, CD8) in addition to the intracellular protein, we recommend that the investigator determine whether the fixation and permeabilization steps adversely affect the surface protein. If so, surface staining of cells prior to fixation and permeabilization is recommended. For intracellular staining, cells must first be fixed and permeabilized. Use of 4% paraformaldehyde in PBS as a fixative is recommended. Other formulations or tissue fixatives may affect the staining properties of the monoclonal antibody. For permeabilization, 0.1% saponin in a balanced salt solution is effective in facilitating antibody entry into cells. Due to the reversible nature of cell membrane permeabilization, saponin must be included in all buffers used (i. e. both the staining and washing steps). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, 0.1% sodium azide
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.