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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ACY1 / Aminoacylase 1 Antibody (clone 1H9) LS‑C173155
Aminoacylase 1 antibody LS-C173155 is an unconjugated mouse monoclonal antibody to human Aminoacylase 1 (ACY1). Validated for Flow and WB.
Catalog
Size
Price
LS-C173155-100
100 µl (0.18 mg/ml)
Unavailable

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Product Description

Aminoacylase 1 antibody LS-C173155 is an unconjugated mouse monoclonal antibody to human Aminoacylase 1 (ACY1). Validated for Flow and WB.
About ACY1 / Aminoacylase 1
ACY1 / Aminoacylase 1 is a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. Q03154 NM_000666 NP_000657.1

ACY1 Antibody, Aminoacylase-1 Antibody, ACY-1 Antibody, ACY1D Antibody, Acylase Antibody, Acylase I Antibody, Aminoacylase 1 Antibody, Aminoacylase Antibody, Aminoacylase I Antibody


Specifications

Target
Human ACY1 / Aminoacylase 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [1H9]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Immunogen
Full length human recombinant protein of human ACY1 (NP_000657) produced in HEK293T cell.
Specificity
Human ACY1 / Aminoacylase
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY1 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY1 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY1 antibody, and then analyzed by flow cytometry.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY1.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY1 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/18/2018

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