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Anti-ACTN4 Antibody (clone 4D10) IHC-plus™ LS-B5374

Note: This antibody replaces LS-C133032

Ordering

Wt. Vol. Conc. Price
50 µg - 0.46 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ACTN4 Antibodies

Anti-ACTN4 Antibody (aa28-38) LS-C20281
Rabbit Polyclonal (IgG) to Human ACTN4
Human, Monkey, Mouse, Rat, Bovine, Dog
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
Anti-ACTN4 Antibody (clone 4D10) IHC-plus™ LS-B5374
Mouse Monoclonal [clone 4D10] (IgG2a,k) to Human ACTN4
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 4D10] (IgG2a,k) to Human ACTN4
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human ACTN4
Mouse
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [4D10]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (20 µg/ml)
  • Western blot
  • ELISA

Specificity and Use

ACTN4 antibody was raised against aCTN4 (NP_004915, 592 a.a. ~ 702 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human ACTN4

Packaging

PBS, pH 7.4. Sourced in Ascites.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About ACTN4

O43707 NM_004924 NP_004915.2

ACTN4 Antibody, ACTININ-4 Antibody, Actinin, alpha 4 Antibody, Actinin alpha4 isoform Antibody, FSGS1 Antibody, F-actin cross-linking protein Antibody, Non-muscle alpha-actinin 4 Antibody, Alpha-actinin-4 Antibody, FSGS Antibody

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane.

Immunohistochemistry

Immunohistochemistry
Anti-ACTN4 / Alpha-Actinin-4 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5374 concentration 5 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to ACTN4 on HeLa cell. [antibody concentration 20 ug/ml]

Western blot

Western blot
Western blot of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody (M01), clone 4D10.

Western blot

Western blot
ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in PC-12.

Western blot

Western blot
ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in NIH/3T3.

Western blot

Western blot
ACTN4 monoclonal antibody (M01), clone 4D10 Western blot of ACTN4 expression in HeLa NE.

Western blot

Western blot
ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in Raw 264.7.

Western blot

Western blot
ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in MCF-7.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged ACTN4 is approximately 0.1 ng/ml as a capture antibody.
 

Requested From: United States
Date Requested: 12/11/2016

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