LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-Actin Antibody LS-C63547

Ordering

Wt. Vol. Conc. Price
- 200 µl - $665
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Actin Antibodies

Anti-Actin Antibody LS-C63547
Mouse Monoclonal (IgG2a)
Human, Mouse, Rat, Guinea pig, Rabbit, Sheep
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-Actin Antibody (N-Terminus, clone EP184E) IHC-plus™ LS-B6276
Rabbit Monoclonal [clone EP184E] (IgG) to Human Actin
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2a)
Human, Mouse, Rat, Guinea pig, Rabbit, Sheep
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Actin
Mouse
Human, Mouse, Rat, Guinea pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
IgG2a Monoclonal
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • IHC - Frozen
  • Immunofluorescence (1:200 - 1:2000)
  • Western blot (1:500)
  • ELISA

Specificity and Use

Actin antibody was raised against synthetic peptide: SGPSIVHALCP (human) attached to Multiple Antigen Peptide (MAP) backbone. Epitope: Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms.
The antibody does not react with the epitope on the N-terminal end of actin. Species cross-reactivity: Cross-reacts with Human, Mouse, Rat, bovine, Carp, Chicken, canine, Frog, Goat, Guinea pig, Hamster, Pig, Rabbit, Sheep, Snail and Viper.
Suitable for use in Immunofluorescence, ELISA, Western Blot Dot Blot and Immunohistochemistry. Immunofluorescence: 1:200-1:2000. Staining of cultured human or chicken fibroblasts. Western Blot: 1:500. Band at ~42kD. Immunohistochemistry (Paraffin): Methanol-fixed sections. Zinc-formalin, B5, ethanol, methacarn, Brunnel's or Bouin's solution can be used as fixatives. Can also be used on frozen sections. Positive control: Human or chicken fibroblast extract.

Packaging

15 mM sodium azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About Actin


Immunohistochemistry

Immunohistochemistry
Human Prostate, Smooth Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
 

Requested From: United States
Date Requested: 12/5/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number