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Anti-Actin Antibody (aa50-70) LS-C63539

Ordering

Wt. Vol. Conc. Price
- 100 µl - $545
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Actin Antibodies

Anti-Actin Antibody LS-C63547
Mouse Monoclonal (IgG2a)
Human, Mouse, Rat, Guinea pig, Rabbit, Sheep
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-Actin Antibody (N-Terminus, clone EP184E) IHC-plus™ LS-B6276
Rabbit Monoclonal [clone EP184E] (IgG) to Human Actin
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1,k) to Chicken Actin
Chicken
IHC - Frozen, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Chicken Actin
Mouse
Chicken (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Frozen
  • Immunofluorescence (1:100)
  • Western blot (1:100 - 1:1000)
  • ELISA

Specificity and Use

Actin antibody was raised against purified chicken gizzard actin.
aa50-70
Appears to be specific for the N-terminal two-thirds of the actin molecule (possibly near residues 50-70 in primary sequence). Reacts with every actin form tested. Species cross-reactivity: Reacts with all animal species and cell types with an actin form, including plant actin.
Suitable for use in Indirect Immunofluorescence, Western Blot, Iodination and Solid Phase Binding. Indirect immunofluorescence: 1:100; Tissue culture cells-fix with formaldehyde, treat with methanol or acetone. Glycerinated myofibrils-fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts. Cryostat sections (6um)-quick frozen in isopentane, slides treated with gelatin and formaldehyde. Western Blots: 1:100-1:1000; On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle. Solid Phase Binding Assay: 1:800-1:1000; In an ELISA, is strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoideum and Physarum polycephalum.

Packaging

0.01% sodium azide.
For research use only.

About Actin


Requested From: United States
Date Requested: 12/3/2016

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