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ACTG1 / Gamma Actin Antibody (N‑Terminus, Biotin) LS‑C143030
Gamma Actin antibody LS-C143030 is a biotin-conjugated rabbit polyclonal antibody to Gamma Actin (ACTG1) from human and mouse. Validated for IP and WB.
Catalog
Size
Price
LS-C143030-100
100 µl
Unavailable

Popular ACTG1 / Gamma Actin Products

Antibody
Species: Human, Mouse, Rat
Applications: IHC, ICC, Western blot
Anti-ACTG1 / Gamma-Actin antibody IHC of human prostate, smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4209 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Methanol fixed human dermal fibroblasts immunostained with 2A3 (1:500)
Species: Human, Mouse, Rat, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA
Anti-ACTG1 / Gamma Actin antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10963 concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-ACTG1 / Gamma Actin antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

Gamma Actin antibody LS-C143030 is a biotin-conjugated rabbit polyclonal antibody to Gamma Actin (ACTG1) from human and mouse. Validated for IP and WB.
About ACTG1 / Gamma Actin
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. P63261 NM_001614 NP_001605.1

ACTG1 Antibody, ACTG Antibody, Actin, gamma 1 Antibody, BRWS2 Antibody, Cytoskeletal gamma-actin Antibody, DFNA20 Antibody, Actin, cytoplasmic 2 Antibody, Gamma-actin Antibody, DFNA26 Antibody


Specifications

Target
Human ACTG1 / Gamma Actin
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with DY650, DY488, DY550.
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:5000 - 1:15000)
  • Immunoprecipitation (1:5000 - 1:15000)
  • (applications tested for the base form of this product only)
Epitope
N-Terminus
Specificity
The epitope recognized by this antibody maps to the N-terminus of human Actin Gamma 1 [UniProt# P63261]. The N-terminus of Actin Gamma 1 is highly conserved with Beta Actin and preliminary indications are that the antibody also recognizes Beta Actin (GeneID 60)."
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunocytochemistry

Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5000 dilution Lane 2 - 1:10000 dilution Lane 3 - 1:15000 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5000 dilution Lane 2 - 1:10000 dilution Lane 3 - 1:15000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5000 dilution Lane 2 - 1:10000 dilution Lane 3 - 1:15000 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5000 dilution Lane 2 - 1:10000 dilution Lane 3 - 1:15000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5000 dilution Lane 2 - 1:10000 dilution Lane 3 - 1:15000 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5000 dilution Lane 2 - 1:10000 dilution Lane 3 - 1:15000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunocytochemistry

Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5000 dilution Lane 2 - 1:10000 dilution Lane 3 - 1:15000 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5000 dilution Lane 2 - 1:10000 dilution Lane 3 - 1:15000 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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