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Anti-ACTB / Beta Actin Antibody (N-Terminus) IHC-plus™ LS-B1625

Note: This antibody replaces LS-C41293


Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular ACTB / Beta Actin Antibodies

Anti-ACTB / Beta Actin Antibody (C-Terminus) IHC-plus™ LS-B1040
Rabbit Polyclonal to Human ACTB / Beta Actin
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot
Immunohistochemistry Image
Anti-ACTB / Beta Actin Antibody (N-Terminus, HRP) LS-C126575
Rabbit Monoclonal (IgG) to Human ACTB / Beta Actin
Human, Monkey, Mouse, Rat, Bovine, Pig
Western blot
HRP Conjugated
Anti-ACTB / Beta Actin Antibody (aa2-16) IHC-plus™ LS-B6503
Rabbit Polyclonal to Human ACTB / Beta Actin
Human, Mouse, Rat
IHC - Paraffin, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (10)
Rabbit Polyclonal (IgG) to Mouse ACTB / Beta Actin
Mouse, Human, Rat
IHC - Paraffin, Western blot


Mouse ACTB / Beta Actin
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Affinity purified


  • IHC - Paraffin (1:100)
  • Western blot

Specificity and Use

ACTB / Beta Actin antibody was raised against synthetic peptide from mouse ACTB / Beta-actin.
Peptide-KLH, NH2 terminus
Immunohistochemistry: LS-B1625 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1625 was determined to be 1:100.


Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (10)

Gap-junctional communication is required for mitotic clonal expansion during adipogenesis. Yanagiya T, Tanabe A, Hotta K. Obesity (Silver Spring, Md.). 2007 15:572-82. [PubMed:17372306]
IL-21 induces inhibitor of differentiation 2 and leads to complete abrogation of anaphylaxis in mice. Kishida T, Hiromura Y, Shin-Ya M, Asada H, Kuriyama H, Sugai M, Shimizu A, Yokota Y, Hama T, Imanishi J, Hisa Y, Mazda O. Journal of immunology (Baltimore, Md. : 1950). 2007 179:8554-61. [PubMed:18056403]
Epoxycholesterol impairs cholesteryl ester hydrolysis in macrophage foam cells, resulting in decreased cholesterol efflux. Ouimet M, Wang MD, Cadotte N, Ho K, Marcel YL. Arteriosclerosis, thrombosis, and vascular biology. 2008 28:1144-50. (WB) [PubMed:18369155]
Activated protein C up-regulates IL-10 and inhibits tissue factor in blood monocytes. Toltl LJ, Beaudin S, Liaw PC. Journal of immunology (Baltimore, Md. : 1950). 2008 181:2165-73. [PubMed:18641355]
Fusion of OTT to BSAC results in aberrant up-regulation of transcriptional activity. Sawada T, Nishiyama C, Kishi T, Sasazuki T, Komazawa-Sakon S, Xue X, Piao JH, Ogata H, Nakayama J, Taki T, Hayashi Y, Watanabe M, Yagita H, Okumura K, Nakano H. The Journal of biological chemistry. 2008 283:26820-8. [PubMed:18667423] [PMC:PMC3258922]
Knockdown of macrophage migration inhibitory factor disrupts adipogenesis in 3T3-L1 cells. Ikeda D, Sakaue S, Kamigaki M, Ohira H, Itoh N, Ohtsuka Y, Tsujino I, Nishimura M. Endocrinology. 2008 149:6037-42. [PubMed:18703634]
Muramyldipeptide augments the actions of lipopolysaccharide in mice by stimulating macrophages to produce pro-IL-1 and by down-regulation of the suppressor of cytokine signaling 1 (SOCS1). Shikama Y, Kuroishi T, Nagai Y, Iwakura Y, Shimauchi H, Takada H, Sugawara S, Endo Y. Innate immunity. 2011 17:15-Mar. [PubMed:19897531]
The pathogen recognition receptor NOD2 regulates human FOXP3+ T cell survival. Rahman MK, Midtling EH, Svingen PA, Xiong Y, Bell MP, Tung J, Smyrk T, Egan LJ, Faubion WA. Journal of immunology (Baltimore, Md. : 1950). 2010 184:7247-56. [PubMed:20483763]
Genetic deletion of the P2Y2 receptor offers significant resistance to development of lithium-induced polyuria accompanied by alterations in PGE2 signaling. Zhang Y, Pop IL, Carlson NG, Kishore BK. American journal of physiology. Renal physiology. 2012 302:F70-7. [PubMed:21975874]
c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis. Piao X, Komazawa-Sakon S, Nishina T, Koike M, Piao JH, Ehlken H, Kurihara H, Hara M, Van Rooijen N, Schtz G, Ohmuraya M, Uchiyama Y, Yagita H, Okumura K, He YW, Nakano H. Science signaling. 2012 5:ra93. [PubMed:23250397]

About ACTB / Beta Actin

P60709 NM_001101 NP_001092.1

ACTB Antibody, Actin, cytoplasmic 1 Antibody, Beta-actin Antibody, Beta actin Antibody, BRWS1 Antibody, Actin, beta Antibody, Beta cytoskeletal actin Antibody, PS1TP5-binding protein 1 Antibody, PS1TP5BP1 Antibody

Actin is a highly conserved protein that participates in a wide variety of cellular functions in eukaryotes including muscle contraction, amoeboid movement, cytokinesis and mitotic division. Beta actin is ubiquitously expressed in all eukaryotic cells.


Anti-Beta Actin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1625 dilution 1:100.


Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.

Western blot

Western blot
Hela cell extract was western blotted with Poly6221. Proteins were visualized using a HRP donkey anti-rabbit antibody and a chemiluminescence detection system.

Requested From: United States
Date Requested: 10/23/2016

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