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ACTB / Beta Actin Antibody (aa1‑14, clone 2A2.1) LS‑C20276
Beta Actin antibody LS-C20276 is an unconjugated mouse monoclonal antibody to xenopus Beta Actin (ACTB). Validated for ELISA, ICC, IF, IHC and WB.
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Review This ProductPopular ACTB / Beta Actin Products
Species:
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep, Frog
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Species:
Mouse, Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Rat, Bovine, Pig
Applications:
Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Product Description
Beta Actin antibody LS-C20276 is an unconjugated mouse monoclonal antibody to xenopus Beta Actin (ACTB). Validated for ELISA, ICC, IF, IHC and WB.
About ACTB / Beta Actin
Actin is a highly conserved protein that participates in a wide variety of cellular functions in eukaryotes including muscle contraction, amoeboid movement, cytokinesis and mitotic division. Beta actin is ubiquitously expressed in all eukaryotic cells.
P60709
NM_001101
NP_001092.1
ACTB Antibody, Actin, cytoplasmic 1 Antibody, Beta-actin Antibody, Beta actin Antibody, BRWS1 Antibody, Actin, beta Antibody, Beta cytoskeletal actin Antibody, PS1TP5-binding protein 1 Antibody, PS1TP5BP1 Antibody
Specifications
Target
Xenopus ACTB / Beta Actin
Host
Mouse
Reactivity
Xenopus
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
[2A2.1]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
- IHC
- ICC
- Immunofluorescence
- Western blot (1:2500)
- ELISA
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Immunogen
Synthetic peptide corresponding to aa1-14 [DDDIAALVIDNGSGL] of Xenopus laevis beta Actin (KLH). Epitope: N-terminal of the beta isoform of actin. Molecular Weight: 42kD. Percent identity by BLAST analysis: Seq squirt, Sea anemone (93%); Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Medaka, Mosquito, Ant (87%).
Epitope
aa1-14
Specificity
Recognizes beta Actin in a wide variety of tissues and species. Does not cross-react with adult cardiac or skeletal muscle or amoeba beta actin. Species cross-reactivity: Human, bovine, Xenopus, Drosophila melanogaster, sheep, porcine, rabbit, feline, canine, mouse, rat, guinea pig, chicken, fish (carp and goldfish) and leech.
Usage
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunofluorescence. Western Blot:1:2500. Useful as a loading control for a wide variety of cell extracts. Immunocytochemistry (acetone-fixed): 1:500. Immunohistochemistry: The epitope recognized by the antibody is resistant to paraffin or formalin-fixed tissue sections. B5, Methacarn, ethanol or Bouin's solution may also be used as fixatives. Immunofluorescence: Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque. Positive control: Cultured human or chicken fibroblasts.
Presentation
15 mM sodium azide, 40% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Requested From:
Date Requested: 6/24/2018
Date Requested: 6/24/2018
