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Anti-ACTB / Beta Actin Antibody (aa1-14, clone 2A2.1) LS-C20276
Most Popular ACTB / Beta Actin Antibodies
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Details
| Xenopus ACTB / Beta Actin | |
| Mouse | |
| Xenopus (tested or 100% immunogen sequence identity) | |
| IgG1 Monoclonal [2A2.1] | |
| Unconjugated | |
| Ascites | |
| Unmodified |
Applications
- IHC
- ICC
- Immunofluorescence
- Western blot (1:2500)
- ELISA
Specificity and Use
| ACTB / Beta Actin antibody was raised against synthetic peptide corresponding to aa1-14 [DDDIAALVIDNGSGL] of Xenopus laevis beta Actin (KLH). Epitope: N-terminal of the beta isoform of actin. Molecular Weight: 42kD. Percent identity by BLAST analysis: Seq squirt, Sea anemone (93%); Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Medaka, Mosquito, Ant (87%). | |
| aa1-14 | |
| Recognizes beta Actin in a wide variety of tissues and species. Does not cross-react with adult cardiac or skeletal muscle or amoeba beta actin. Species cross-reactivity: Human, bovine, Xenopus, Drosophila melanogaster, sheep, porcine, rabbit, feline, canine, mouse, rat, guinea pig, chicken, fish (carp and goldfish) and leech. | |
| Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunofluorescence. Western Blot:1:2500. Useful as a loading control for a wide variety of cell extracts. Immunocytochemistry (acetone-fixed): 1:500. Immunohistochemistry: The epitope recognized by the antibody is resistant to paraffin or formalin-fixed tissue sections. B5, Methacarn, ethanol or Bouin's solution may also be used as fixatives. Immunofluorescence: Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque. Positive control: Cultured human or chicken fibroblasts. |
Packaging
| 15 mM sodium azide, 40% glycerol. | |
| Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. | |
| For research use only. |
About ACTB / Beta Actin
P60709
NM_001101
NP_001092.1
ACTB Antibody, Actin, cytoplasmic 1 Antibody, Beta-actin Antibody, Beta actin Antibody, BRWS1 Antibody, Actin, beta Antibody, Beta cytoskeletal actin Antibody, PS1TP5-binding protein 1 Antibody, PS1TP5BP1 Antibody
Actin is a highly conserved protein that participates in a wide variety of cellular functions in eukaryotes including muscle contraction, amoeboid movement, cytokinesis and mitotic division. Beta actin is ubiquitously expressed in all eukaryotic cells.
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Date Requested: 6/28/2017
Date Requested: 6/28/2017
