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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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ACTB / Beta Actin Antibody (aa1‑14, clone 2A2.1) LS‑C20276
Antibody
Beta Actin antibody LS-C20276 is an unconjugated mouse monoclonal antibody to xenopus Beta Actin (ACTB). Validated for ELISA, ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C20276-100
100 µl
Unavailable

Popular ACTB / Beta Actin Products

Anti-Beta Actin Antibody. Tissue: sections 4.2 mm thick of rana pipiens tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Beta Actin antibody at 1:200 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.
Species: Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep, Frog
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-Beta Actin antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1040 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
Anti-Beta Actin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1625 dilution 1:100.
Species: Mouse, Human, Rat
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Pig
Applications: Western blot
Anti-Beta Actin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6503 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Beta Actin antibody LS-C20276 is an unconjugated mouse monoclonal antibody to xenopus Beta Actin (ACTB). Validated for ELISA, ICC, IF, IHC and WB.
About ACTB / Beta Actin
Actin is a highly conserved protein that participates in a wide variety of cellular functions in eukaryotes including muscle contraction, amoeboid movement, cytokinesis and mitotic division. Beta actin is ubiquitously expressed in all eukaryotic cells. P60709 NM_001101 NP_001092.1

ACTB Antibody, Actin, cytoplasmic 1 Antibody, Beta-actin Antibody, Beta actin Antibody, BRWS1 Antibody, Actin, beta Antibody, Beta cytoskeletal actin Antibody, PS1TP5-binding protein 1 Antibody, PS1TP5BP1 Antibody


Specifications

Target
Xenopus ACTB / Beta Actin
Host
Mouse
Reactivity
Xenopus (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [2A2.1]
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • ICC
  • Immunofluorescence
  • Western blot (1:2500)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide corresponding to aa1-14 [DDDIAALVIDNGSGL] of Xenopus laevis beta Actin (KLH). Epitope: N-terminal of the beta isoform of actin. Molecular Weight: 42kD. Percent identity by BLAST analysis: Seq squirt, Sea anemone (93%); Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Medaka, Mosquito, Ant (87%).
Epitope
aa1-14
Specificity
Recognizes beta Actin in a wide variety of tissues and species. Does not cross-react with adult cardiac or skeletal muscle or amoeba beta actin. Species cross-reactivity: Human, bovine, Xenopus, Drosophila melanogaster, sheep, porcine, rabbit, feline, canine, mouse, rat, guinea pig, chicken, fish (carp and goldfish) and leech.
Usage
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunofluorescence. Western Blot:1:2500. Useful as a loading control for a wide variety of cell extracts. Immunocytochemistry (acetone-fixed): 1:500. Immunohistochemistry: The epitope recognized by the antibody is resistant to paraffin or formalin-fixed tissue sections. B5, Methacarn, ethanol or Bouin's solution may also be used as fixatives. Immunofluorescence: Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque. Positive control: Cultured human or chicken fibroblasts.
Presentation
15 mM sodium azide, 40% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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