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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ACTA1 / Skeletal Muscle Actin Antibody (N‑Terminus, Azide‑free) LS‑C34838
Antibody
Skeletal Muscle Actin antibody LS-C34838 is an unconjugated mouse monoclonal antibody to Skeletal Muscle Actin (ACTA1) from human, rat, bovine and other species. Validated for IF and IHC.
Catalog
Size
Price
LS-C34838-100
100 µg (0.2 mg/ml)
Unavailable

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Product Description

Skeletal Muscle Actin antibody LS-C34838 is an unconjugated mouse monoclonal antibody to Skeletal Muscle Actin (ACTA1) from human, rat, bovine and other species. Validated for IF and IHC.
About ACTA1 / Skeletal Muscle Actin
Actin alpha 1 which is expressed in skeletal muscle is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. P68133 NM_001100 NP_001091.1

ACTA1 Antibody, ACTA Antibody, Actin, alpha skeletal muscle Antibody, Alpha-actin-1 Antibody, CFTD Antibody, CFTD1 Antibody, CFTDM Antibody, MPFD Antibody, NEM1 Antibody, NEM2 Antibody, ASMA Antibody, NEM3 Antibody


Specifications

Target
Human ACTA1 / Skeletal Muscle Actin
Host
Mouse
Reactivity
Human, Rat, Bovine, Guinea pig, Rabbit, Sheep, Frog (tested or 100% immunogen sequence identity)
Clonality
IgM,k Monoclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Azide-free
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
N-terminal peptide of alpha skeletal muscle actin; acetylated at the N-terminus and conjugated to KLH at the C-terminus. Cellular Localization: Cytoplasmic. Epitope: N-terminal decapeptide.
Epitope
N-Terminus
Specificity
Recognizes human alpha-skeletal and alpha-cardiac (sarcomeric) actins as well as neoplasms derived from normal tissues (i. e. rhabdomyosarcomas). Shows no cross-reaction with smooth muscle actin. Species cross-reactivity: bovine, rabbit, sheep, rat, guinea pig, and frog.
Usage
Suitable for use in Immunofluorescence and Immunohistochemistry. Immunohistochemistry: 2-4 ug/ml for 30 min. at RT. Formalin/paraffin: No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. Positive control: Skeletal or Cardiac muscle or Rhabdomyosarcoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/22/2018

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