Human ACTA1 / Skeletal Muscle Actin
Human, Rat, Bovine, Guinea pig, Rabbit, Sheep, Frog
(tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
- IHC - Paraffin
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N-terminal peptide of alpha skeletal muscle actin; acetylated at the N-terminus and conjugated to KLH at the C-terminus. Cellular Localization: Cytoplasmic. Epitope: N-terminal decapeptide.
Recognizes human alpha-skeletal and alpha-cardiac (sarcomeric) actins as well as neoplasms derived from normal tissues (i. e. rhabdomyosarcomas). Shows no cross-reaction with smooth muscle actin. Species cross-reactivity: bovine, rabbit, sheep, rat, guinea pig, and frog.
Suitable for use in Immunofluorescence and Immunohistochemistry. Immunohistochemistry: 2-4 ug/ml for 30 min. at RT. Formalin/paraffin: No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. Positive control: Skeletal or Cardiac muscle or Rhabdomyosarcoma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, pH 7.4.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.