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Anti-ACSL4 / FACL4 Antibody (N-Terminus) IHC-plus™ LS-B5818

Note: This antibody replaces LS-C81454

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ACSL4 / FACL4 Antibodies

Anti-ACSL4 / FACL4 Antibody (aa658-670) IHC-plus™ LS-B4440
Goat Polyclonal to Human ACSL4 / FACL4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ACSL4 / FACL4 Antibody (N-Terminus) IHC-plus™ LS-B5818
Rabbit Polyclonal (IgG) to Human ACSL4 / FACL4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ACSL4 / FACL4 Antibody (aa244-274, Azide-free, HRP) LS-C212589
Rabbit Polyclonal (IgG) to Human ACSL4 / FACL4
Human
IHC, ICC, Immunofluorescence, Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human ACSL4 / FACL4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ACSL4 / FACL4
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Chicken, Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ACSL4 / FACL4 antibody was raised against synthetic peptide from N-Terminus of human ACSL4 (O60488, NP_004449). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Opossum, Turkey, Zebra finch, Chicken, Xenopus (92%); Platypus (85%).
LS-E17970 - Lyophilized - 100 µg - $145.00
N-Terminus
Human ACSL4 / FACL4
ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ACSL4 / FACL4

O60488 NM_004458 NP_004449.1

ACSL4 Antibody, Acyl-CoA synthetase 4 Antibody, ACS4 Antibody, FACL4 Antibody, LACS4 Antibody, LACS 4 Antibody, Lignoceroyl-CoA synthase Antibody, MRX63 Antibody, MRX68 Antibody

ACSL4 / FACL4 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome.

Immunohistochemistry

Immunohistochemistry
Anti-ACSL4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5818 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/11/2016

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