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Anti-ACSL4 / FACL4 Antibody (aa658-670) IHC-plus™ LS-B4440

Note: This antibody replaces LS-C54808

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ACSL4 / FACL4 Antibodies

Anti-ACSL4 / FACL4 Antibody (aa658-670) IHC-plus™ LS-B4440
Goat Polyclonal to Human ACSL4 / FACL4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ACSL4 / FACL4 Antibody (N-Terminus) IHC-plus™ LS-B5818
Rabbit Polyclonal (IgG) to Human ACSL4 / FACL4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ACSL4 / FACL4 Antibody (aa244-274, Azide-free, HRP) LS-C212589
Rabbit Polyclonal (IgG) to Human ACSL4 / FACL4
Human
IHC, ICC, Immunofluorescence, Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human ACSL4 / FACL4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ACSL4 / FACL4
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Chicken (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.1 - 0.3 µg/ml)
  • ELISA (1:128000)

Specificity and Use

ACSL4 / FACL4 antibody was raised against synthetic peptide C-HYLKDIERMYGGK from the C-terminus of human ACSL4 / FACL4 (NP_004449.1; NP_075266.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%); Opossum, Turkey, Chicken, Platypus, Pufferfish (92%); Xenopus, Stickleback, Zebrafish (85%).
LS-E25435 - Lyophilized - 100 µg - $145.00
aa658-670
Human ACSL4 / FACL4. This antibody is expected to recognise isoform 1 (NP_004449.1) and isoform 2 (NP_075266.1).
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 80 kDa band observed in lysates of cell line HepG2 (calculated MW of 79.2 kDa according to NP_075266.1). Recommended concentration: 0.1-0.3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About ACSL4 / FACL4

O60488 NM_004458 NP_004449.1

ACSL4 Antibody, Acyl-CoA synthetase 4 Antibody, ACS4 Antibody, FACL4 Antibody, LACS4 Antibody, LACS 4 Antibody, Lignoceroyl-CoA synthase Antibody, MRX63 Antibody, MRX68 Antibody

ACSL4 / FACL4 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome.

Immunohistochemistry

Immunohistochemistry
Anti-ACSL4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4440 concentration 3.75 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-ACSL4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4440 concentration 3.75 ug/ml.

Western blot

Western blot
ACSL4 antibody (0.1 ug/ml) staining of HepG2 lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/9/2016

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