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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ACS5 / ACSL5 Antibody (aa470‑519) IHC‑plus™ LS‑B5798
Note: This antibody replaces LS-C81011
ACSL5 antibody LS-B5798 is an unconjugated rabbit polyclonal antibody to ACSL5 (ACS5) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B5798-50
50 µg (1 mg/ml)
Unavailable

Popular ACS5 / ACSL5 Products

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Antibody
Species: Human
Applications: IHC, Western blot

Product Description

ACSL5 antibody LS-B5798 is an unconjugated rabbit polyclonal antibody to ACSL5 (ACS5) from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About ACS5 / ACSL5
Acyl-CoA synthetases (ACSL) activate long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage (By similarity). Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids (By similarity). It was suggested that it may also stimulate fatty acid oxidation (By similarity). Q9ULC5 NM_016234 NP_057318.2

ACSL5 Antibody, ACS2 Antibody, ACS5 Antibody, FACL5 Antibody, Fatty acid coenzyme A ligase 5 Antibody, Phosphatidylinositol 4-kinase Antibody, LACS 5 Antibody


Specifications

Target
Human ACS5 / ACSL5
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat, Bovine, Dog, Chicken, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ACS5 / ACSL5 antibody was raised against synthetic peptide located between aa470-519 of human ACSL5 (Q9ULC5, NP_976313). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat (100%); Galago, Dog, Bovine, Bat, Turkey, Zebra finch, Chicken, Platypus, Lizard, Seabass, Zebrafish (92%); Gibbon, Elephant, Rabbit, Pig, Opossum, Guinea pig, Armadillo, Salmon (85%); Panda, Horse, Stickleback, Slime mold (84%).
Blocking Peptide
LS-E17129 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5798. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa470-519
Specificity
Human ACSL5
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-ACSL5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5798 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ACSL5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5798 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ACSL5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5798 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ACSL5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5798 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ACSL5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5798 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ACSL5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5798 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-ACSL5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5798 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ACSL5 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5798 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Placenta lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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