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Anti-Acrosin Antibody (aa99-127) LS-C155966

Ordering

Wt. Vol. Conc. Price
- 400 µl - Unavailable

Most Popular Acrosin Antibodies

Anti-Acrosin Antibody (aa231-280) LS-C145372
Rabbit Polyclonal to Human Acrosin
Human, Bovine, Horse, Sheep
Western blot
Unconjugated
Western blot Image
Anti-Acrosin Antibody (aa99-127) LS-C155966
Rabbit Polyclonal to Human Acrosin
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-Acrosin Antibody (aa387-416) IHC-plus™ LS-B10478
Rabbit Polyclonal to Human Acrosin
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human Acrosin
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human Acrosin
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

LS-E9929 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C155966. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa99-127
This ACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-127 amino acids from the Central region of human ACR.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About Acrosin

P10323 NM_001097 NP_001088.2

ACR Antibody, ACRS Antibody, Acrosin Antibody, Proacrosin Antibody, Preproacrosin Antibody

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human testis tissue reacted with ACR Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot
Western blot of ACR Antibody in T47D cell line lysates (35 ug/lane). ACR (arrow) was detected using the purified antibody.

Flow Cytometry

Flow Cytometry
ACR Antibody flow cytometry of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

Requested From: 
Date Requested: 3/28/2017

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