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Anti-ACKR1 / DARC Antibody (aa6-24) IHC-plus™ LS-B2393

Note: This antibody replaces LS-C54742, LS-C64509

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ACKR1 / DARC Antibodies

Anti-ACKR1 / DARC Antibody (C-Terminus) IHC-plus™ LS-B2306
Rabbit Polyclonal to Human ACKR1 / DARC
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ACKR1 / DARC Antibody (aa1-30, FITC) LS-C222003
Rabbit Polyclonal (IgG) to Human ACKR1 / DARC
Human
Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human ACKR1 / DARC
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human ACKR1 / DARC
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:16000) & (0.1 - 0.3 µg/ml)
  • ELISA (1:16000)

Specificity and Use

ACKR1 / DARC antibody was raised against synthetic peptide HRAELSPSTENSSQLDFED-C from the N-terminus of human DARC (NP_001116423.1; NP_002027.2). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey (100%); Gorilla (95%); Gibbon (89%); Marmoset (84%).
LS-E25954 - Lyophilized - 100 µg - $145.00
aa6-24
Human DARC. This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2).
Immunohistochemistry: LS-B2393 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2393 was determined to be 2.5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About ACKR1 / DARC

Q16570 NM_002036 NP_002027.2

ACKR1 Antibody, CD234 Antibody, CD234 antigen Antibody, DARC Antibody, Duffy antigen Antibody, Duffy blood group Antibody, Duffy group antigen Antibody, Dfy Antibody, FY Antibody, Fy glycoprotein Antibody, Plasmodium vivax receptor Antibody, WBCQ1 Antibody, CCBP1 Antibody, Duffy blood group antigen Antibody, Glycoprotein D Antibody, GPD Antibody, GpFy Antibody

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor.

Immunohistochemistry

Immunohistochemistry
Anti-DARC antibody IHC of human lung, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2393 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.1 ug/ml) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/7/2016

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