Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
ACIN1 / Acinus antibody was raised against 6HIS fusion protein corresponding to residues 1233-1341 of human Acinus-L.
Recognizes Acinus-L, Acinus-S and Acinus-S', Mr ~220, 98 and 94kD respectively. Nonspecific proteins were also detected, Mr ~60 and 50kD. Human. Others not tested.
Suitable for use in immunoblot Analysis. Western Blot Analysis: 0.5-1 ug/ml detects Acinus-L, Acinus-S and Acinus-S' in RIPA lysates from Jurkat cells. Detection of Acinus-L was lost in Jurkat cells treated with 0.5 uM staurosporin for 6 hours. Lysates from untreated and staurosporin treated Jurkat cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Acinus, CT (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Acinus-L, Acinus-S and Acinus-S' (220, 98 and 94kD respectively) with the loss of Acinus-L upon induction of apoptosis. Included Positive Antigen Control: Jurkat lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.