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Anti-ACIN1 / Acinus Antibody (aa1233-1341) LS-C11740

Ordering

Wt. Vol. Conc. Price
200 µg - - $590
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ACIN1 / Acinus Antibodies

Anti-ACIN1 / Acinus Antibody (C-Terminus) LS-C328
Rabbit Polyclonal to Mouse ACIN1 / Acinus
Mouse, Human
IHC, ICC, Western blot
Unconjugated
Immunocytochemistry Image
Anti-ACIN1 / Acinus Antibody (aa775-789) IHC-plus™ LS-B3190
Rabbit Polyclonal to Human ACIN1 / Acinus
Human, Mouse
IHC - Paraffin, ICC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human ACIN1 / Acinus
Human
Western blot
Unconjugated

Details

Human ACIN1 / Acinus
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

Western blot

Specificity and Use

ACIN1 / Acinus antibody was raised against 6HIS fusion protein corresponding to residues 1233-1341 of human Acinus-L.
aa1233-1341
Recognizes Acinus-L, Acinus-S and Acinus-S', Mr ~220, 98 and 94kD respectively. Nonspecific proteins were also detected, Mr ~60 and 50kD. Human. Others not tested.
Suitable for use in immunoblot Analysis. Western Blot Analysis: 0.5-1 ug/ml detects Acinus-L, Acinus-S and Acinus-S' in RIPA lysates from Jurkat cells. Detection of Acinus-L was lost in Jurkat cells treated with 0.5 uM staurosporin for 6 hours. Lysates from untreated and staurosporin treated Jurkat cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Acinus, CT (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Acinus-L, Acinus-S and Acinus-S' (220, 98 and 94kD respectively) with the loss of Acinus-L upon induction of apoptosis. Included Positive Antigen Control: Jurkat lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About ACIN1 / Acinus

Q9UKV3 NM_014977 NP_055792.1

ACIN1 Antibody, ACINUS Antibody, ACN Antibody, KIAA0670 Antibody, FSAP152 Antibody

Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.

Requested From: United States
Date Requested: 12/8/2016

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