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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ACIN1 / Acinus Antibody (aa1233-1341)

Acinus antibody LS-C11740 is an unconjugated rabbit polyclonal antibody to human Acinus (ACIN1). Validated for WB.
Catalog
Size
Price
LS-C11740-200
200 µg
$680
Description
Acinus antibody LS-C11740 is an unconjugated rabbit polyclonal antibody to human Acinus (ACIN1). Validated for WB.
Target
Human ACIN1 / Acinus
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
ACIN1 / Acinus antibody was raised against 6HIS fusion protein corresponding to residues 1233-1341 of human Acinus-L.
Epitope
aa1233-1341
Specificity
Recognizes Acinus-L, Acinus-S and Acinus-S', Mr ~220, 98 and 94kD respectively. Nonspecific proteins were also detected, Mr ~60 and 50kD. Human. Others not tested.
Usage
Suitable for use in immunoblot Analysis. Western Blot Analysis: 0.5-1 ug/ml detects Acinus-L, Acinus-S and Acinus-S' in RIPA lysates from Jurkat cells. Detection of Acinus-L was lost in Jurkat cells treated with 0.5 uM staurosporin for 6 hours. Lysates from untreated and staurosporin treated Jurkat cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Acinus, CT (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Acinus-L, Acinus-S and Acinus-S' (220, 98 and 94kD respectively) with the loss of Acinus-L upon induction of apoptosis. Included Positive Antigen Control: Jurkat lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About ACIN1 / Acinus
Q9UKV3 NM_014977 NP_055792.1

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Requested From: United States
Date Requested: 1/21/2019
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