Acinus antibody LS-C11740 is an unconjugated rabbit polyclonal antibody to human Acinus (ACIN1). Validated for WB.
Human ACIN1 / Acinus
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
ACIN1 / Acinus antibody was raised against 6HIS fusion protein corresponding to residues 1233-1341 of human Acinus-L.
Recognizes Acinus-L, Acinus-S and Acinus-S', Mr ~220, 98 and 94kD respectively. Nonspecific proteins were also detected, Mr ~60 and 50kD. Human. Others not tested.
Suitable for use in immunoblot Analysis. Western Blot Analysis: 0.5-1 ug/ml detects Acinus-L, Acinus-S and Acinus-S' in RIPA lysates from Jurkat cells. Detection of Acinus-L was lost in Jurkat cells treated with 0.5 uM staurosporin for 6 hours. Lysates from untreated and staurosporin treated Jurkat cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Acinus, CT (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Acinus-L, Acinus-S and Acinus-S' (220, 98 and 94kD respectively) with the loss of Acinus-L upon induction of apoptosis. Included Positive Antigen Control: Jurkat lysate. Add 2.5 ul of 2-mercaptoethanol/100 ul of lysate and boil for 5 minutes to reduce the preparation. Load 20 ug of reduced lysate per lane for minigels.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.