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Catalog Number	Size	Price
LS-C313073-10	10 µg	$318
LS-C313073-100	100 µg (0.5 mg/ml)	$470

ACCN3 / ASIC3 Antibody - Western blot analysis of ASIC3 using anti-ASIC3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Testis Tissue Lysate, Lane 3: U87 Cell Lysate, Lane 4: NEURO Cell Lysate, Lane 5: SMMC Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ASIC3 at approximately 69KD. The expected band size for ASIC3 is at 58KD.

ACCN3 / ASIC3 Antibody - WB of ACCN3 / ASIC3 antibody. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Testis Tissue Lysate. Lane 3: U87 Cell Lysate. Lane 4: NEURO Cell Lysate. Lane 5: SMMC Cell Lysate.

ACCN3 / ASIC3 Antibody - Flow Cytometry analysis of U937 cells using anti-ASIC3 antibody. Overlay histogram showing U937 cells stained with anti-ASIC3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASIC3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human ACCN3 / ASIC3 Antibody (aa56‑73, WB) LS‑C313073

Name: Anti-ACCN3 / ASIC3 Antibody | Rabbit anti-Human Polyclonal WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C313073-10
Price: 318.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Note: This antibody replaces LS-C388036

Antibody:

ACCN3 / ASIC3 Rabbit anti-Human Polyclonal (aa56-73) Antibody

Application:

Reactivity:

Human, Monkey, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C313073-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ACCN3 / ASIC3 Rabbit anti-Human Polyclonal (aa56-73) Antibody

Application:

Reactivity:

Human, Monkey, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

ASIC3 antibody LS-C313073 is an unconjugated rabbit polyclonal antibody to ASIC3 (ACCN3) (aa56-73) from human. It is reactive with human, mouse, rat and other species. Validated for WB. Cited in 1 publication.

(1) Publication(s)

Target

Human ACCN3 / ASIC3

Synonyms

See All ACCN3 / ASIC3 Antibodies

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the N-Terminus of human ASIC3(56-73 aa FLYQVAERVRYYREFHHQ), different from the related rat and mouse sequences by two amino acids.

Epitope

aa56-73

Specificity

Expressed by sensory neurons. Strongly expressed in brain, spinal chord, lung, lymph nodes, kidney, pituitary, heart and testis. .

Applications

Western blot

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ACCN3 / ASIC3

Uniprot: Q9UHC3 NCBI: NM_020321 NP_064717.1

LSBio Ratings

ACCN3 / ASIC3 Antibody (aa56-73) for WB/Western LS-C313073 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Endomorphins potentiate acid-sensing ion channel currents and enhance the lactic acid-mediated increase in arterial blood pressure: effects amplified in hindlimb ischaemia. Mohamed Farrag, Julie K Drobish, Henry L Puhl, Joyce S Kim, Paul B Herold, Marc P Kaufman, Victor Ruiz-Velasco. The Journal of physiology. 2017 December;595:7167-7183.

Full Text Article PubMed: 29044528 PMC: PMC5709321

Submit a Publication for LS-C313073