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Anti-ACAT1 Antibody (aa143-192) LS-C116567

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

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34 ACAT1 Antibodies

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Anti-ACAT1 Antibody (aa218-267) IHC-plus™ LS-B4679
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Anti-ACAT1 Antibody (N-Terminus, clone AT1.H11) IHC-plus™ LS-B6358
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Anti-ACAT1 Antibody (aa296-329) IHC-plus™ LS-B6919
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Anti-ACAT1 Antibody (aa190-270) LS-C381872
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ACAT1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ACAT1
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ACAT1 antibody was raised against synthetic peptide located between aa143-192 of human ACAT1 (P24752, NP_000010). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Chicken, Xenopus, Salmon, Zebrafish (100%); Smelt, Stickleback (92%); Sea squirt (91%).
LS-E19523 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116567. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa143-192
Human ACAT1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ACAT1

P24752 NM_000019 NP_000010.1

ACAT1 Antibody, ACAT Antibody, Acetoacetyl-CoA thiolase Antibody, Acetyl-CoA acetyltransferase 1 Antibody, MAT Antibody, T2 Antibody, THIL Antibody

The ACAT1 mRNA encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The ACAT gene spans approximately 27 kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Western blot

Western blot
ACAT1 antibody LS-C116567 Western blot of HepG2 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/26/2017

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