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Anti-ABL1 / c-ABL Antibody (clone 8E9., Azide-free) IHC-plus™ LS-B2776

Note: This antibody replaces LS-C12197, LS-C88556

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ABL1 / c-ABL Antibodies

Anti-ABL1 / c-ABL Antibody (clone 8E9., Azide-free) IHC-plus™ LS-B2776
Mouse Monoclonal [clone 8E9.] (IgG1) to Human ABL1 / c-ABL
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated, Azide-free
Immunohistochemistry Image
Anti-ABL1 / c-ABL Antibody (phospho-Tyr412) LS-C178071
Rabbit Polyclonal to Human ABL1 / c-ABL
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ABL1 / c-ABL Antibody (aa612-642, Azide-free, Biotin) LS-C250586
Rabbit Polyclonal (IgG) to Mouse ABL1 / c-ABL
Mouse
IHC, Western blot, ELISA
Biotin Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 8E9.] (IgG1) to Human ABL1 / c-ABL
Human, Mouse
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated, Azide-free

Details

Human ABL1 / c-ABL
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [8E9.]
Unconjugated
Protein G purified
Azide-free

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence
  • Western blot (1 µg/ml)
  • Immunoprecipitation (2 µg/tst)

Specificity and Use

ABL1 / c-ABL antibody was raised against recombinant Abl protein.
Recognizes human c-Abl p120 and chimeric Bcr/Abl proteins. Species cross-reactivity: mouse.
Immunohistochemistry: LS-B2776 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2776 was determined to be 10 ug/ml. Immunoprecipitation: 2 ug/mg protein lysate. Use Protein G. Western Blot: 1 ug/ml for 2hrs at RT. Positive control: HeLa cells or placenta. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Packaging

PBS, pH 7.4.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ABL1 / c-ABL

P00519 NM_005157 NP_005148.2

ABL1 Antibody, Bcr/c-abl oncogene protein Antibody, Bcr-Abl Antibody, Bcr/abl Antibody, C-ABL Antibody, ABL Antibody, JTK7 Antibody, p150 Antibody, Tyrosine-protein kinase ABL1 Antibody, Proto-oncogene c-Abl Antibody, V-abl Antibody

Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis.

Immunohistochemistry

Immunohistochemistry
Anti-ABL1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2776 concentration 10 ug/ml. This image was taken for the unmodified form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/9/2016

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